SOD1 antibody (AA 14-148)
-
- Target See all SOD1 Antibodies
- SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
-
Binding Specificity
- AA 14-148
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This SOD1 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Cu-Zn superoxide dismutase-1 (SOD-1) is a well-characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Enzymatically, SOD-1 facilitates the dismutation of oxygen radicals to hydrogen peroxide and also catalyzes pro-oxidant reactions, which include the peroxidase activity and hydroxyl radical generating activity. SOD-1 is ubiquitously expressed in somatic cells and functions as a homodimer. Defects in the gene encoding SOD-1 have been implicated in the progression of neurological diseases, including amyotrophic lateral sclerosis (ALS), a neurodegenerative disease characterized by the loss of spinal motor neurons, Down syndrome and Alzheimer s disease. In familial ALS, several mutations in SOD-1 predominate, resulting in the loss of zinc binding, the loss of scavenging activity of SOD-1, and correlate with an increase in neurotoxicity and motor neuron death.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa14-148) of human SOD1 (exact sequence is proprietary)
- Isotype
- IgG1
- Top Product
- Discover our top product SOD1 Primary Antibody
-
-
- Application Notes
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: JEG-3 or Jurkat cells. Human breast or ovarian carcinoma.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
- Alternative Name
- SOD1 (SOD1 Products)
- Synonyms
- LOC692639 antibody, ALS antibody, ALS1 antibody, IPOA antibody, SOD antibody, hSod1 antibody, homodimer antibody, CG11793 antibody, Cu antibody, Cu-Zn SOD antibody, Cu/Zn SOD antibody, Cu/Zn sod antibody, Cu/Zn superoxide dismutase antibody, Cu/ZnSOD antibody, CuSOD antibody, CuZn SOD antibody, CuZn-SOD antibody, CuZn-SOD1 antibody, CuZnSOD antibody, Cu[2+]/Zn[2+]SOD antibody, Dmel\\CG11793 antibody, G antibody, Mn SOD antibody, SOD-1 antibody, SOD1 antibody, Sod-1 antibody, Sod1 antibody, To antibody, To-1 antibody, Zn SOD antibody, Zn Sod antibody, Zn-SOD antibody, ZnSod antibody, cSOD antibody, cSod antibody, dSOD1 antibody, l(3)108 antibody, l(3)68Af' antibody, l(3)G antibody, sod antibody, sod1 antibody, CU/ZN-SOD antibody, SODC antibody, DKFZP469M1833 antibody, B430204E11Rik antibody, Cu/Zn-SOD antibody, Ipo-1 antibody, Ipo1 antibody, SOD1L1 antibody, XSODB antibody, als antibody, als1 antibody, ipoa antibody, sod1-a antibody, ZSOD antibody, cuzn antibody, Cu/Zn superoxide dismutase antibody, superoxide dismutase 1 antibody, Superoxide dismutase 1 antibody, superoxide dismutase 1, soluble antibody, superoxide dismutase 1 S homeolog antibody, Superoxide dismutase [Cu-Zn] antibody, superoxide dismutase 1 L homeolog antibody, superoxide dismutase [Cu-Zn]-like antibody, superoxide dismutase [Cu-Zn] antibody, superoxide dismutase Sod1 antibody, SOD antibody, SOD1 antibody, Sod1 antibody, sod1 antibody, sod1.S antibody, sod-1 antibody, sod1.L antibody, LOC101451855 antibody, LOC101115136 antibody
- Background
-
Amyotrophic lateral sclerosis 1 (ALS1), Cu/Zn SOD, Cu/Zn Superoxide Dismutase, Epididymis Secretory Protein Li 44, Indophenoloxidase A (IPOA), Superoxide Dismutase [Cu-Zn], Superoxide Dismutase 1 (SOD1),Superoxide Dismutase 1 (SOD1) (Antioxidant Enzyme)
Cellular localisation: Cytoplasmic - Molecular Weight
- 16kDa
- Gene ID
- 6647, 443914
- UniProt
- P00441
- Pathways
- Sensory Perception of Sound, Transition Metal Ion Homeostasis
-