TNFAIP3 antibody (AA 241-356)

Catalog No. ABIN7127007

Product Details anti-TNFAIP3 Antibody

Target: TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))

Binding Specificity: AA 241-356

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFAIP3 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. HighA20expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade). These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 241-356) of human TNFAIP3 protein (exact sequence is proprietary)

Isotype: IgG2

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Hodgkin s Lymphoma or hepatocellular carcinoma (HCC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for TNFAIP3

Target: TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))

Alternative Name: TNFAIP3 (TNFAIP3 Products)

Synonyms: tnfaip3 antibody, MGC82339 antibody, TNFAIP3 antibody, A20 antibody, OTUD7C antibody, TNFA1P2 antibody, Tnfip3 antibody, TNF alpha induced protein 3 antibody, TNF alpha induced protein 3 S homeolog antibody, tumor necrosis factor, alpha-induced protein 3 antibody, TNFAIP3 antibody, tnfaip3.S antibody, Tnfaip3 antibody

Background: A20, Putative DNA-binding protein A20, TNF alpha-induced protein 3 (TNFAIP3), Tumor Necrosis Factor alpha-Induced Protein 3(TNFAIP3), Zinc finger protein A20,A20 / TNFAIP3 (Marker of Tamoxifen Resistance)
Cellular localisation: Cytoplasmic, Cell Surface and Nuclear

Molecular Weight: 90kDa

Gene ID: 7128, 211600

UniProt: P21580

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response