E2F1 antibody (pThr433)

Catalog No. ABIN7138851

Product Details anti-E2F1 Antibody

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Binding Specificity: pThr433

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Threonine 433(D-L-T(p)-P-L) derived from Human E2F1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1 (E2F1 Products)

Synonyms: CG6376 antibody, DRTF1/E2F antibody, DmE2F-1 antibody, Dmel\\CG6376 antibody, Dp antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E(var)3-95E antibody, E(var)93E antibody, E2F antibody, E2F-1 antibody, E2F1 antibody, E2f1 antibody, Evar(3)164 antibody, dE2F antibody, dE2F1 antibody, dE2f antibody, dE2f1 antibody, de2f1 antibody, def21 antibody, drosE2F1 antibody, e2f antibody, e2f1 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, RBAP1 antibody, RBBP3 antibody, RBP3 antibody, mKIAA4009 antibody, xE2F antibody, ATE2FB antibody, E2F TRANSCRIPTION FACTOR-1 E2F1 antibody, E2F transcription factor 1 antibody, E2FB antibody, E2FB TRANSCRIPTION FACTOR antibody, E2F transcription factor 1 antibody, E2F transcription factor 1 L homeolog antibody, E2f1 antibody, E2F1 antibody, e2f1.L antibody

Background:

Background: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis.

Aliases: Dmel\CG6376 antibody, Dmel_CG6376 antibody, drosE2F1 antibody, E(Sev-CycE)3A antibody, E(var)3-93E antibody, E2-promoter binding facto antibody, E2F 1 antibody, E2F transcription factor 1 antibody, E2F-1 antibody, E2f-PA antibody, E2f-PB antibody, E2f-PC antibody, E2F1 antibody, E2f1 E2F transcription factor 1 antibody, E2F1_HUMAN antibody, Evar(3)164 antibody, KIAA4009 antibody, l(3)07172 antibody, l(3)j3B1 antibody, l(3)j3C2 antibody, l(3)rM729 antibody, mKIAA4009 antibody, OTTHUMP00000030661 antibody, PBR3 antibody, PRB binding protein E2F 1 antibody, PRB-binding protein E2F-1 antibody, RBAP 1 antibody, RBAP-1 antibody, RBAP1 antibody, RBBP-3 antibody, RBBP3 antibody, RBP 3 antibody, RBP3 antibody, Retinoblastoma-associated protein 1 antibody, Retinoblastoma-binding protein 3 antibody, Transcription factor E2F1 antibody

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy