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Pkc beta 1 antibody (pSer642) (Cy3)

Pkc beta 1 Reactivity: Rat, Human, Mouse WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal Cy3
Catalog No. ABIN711464
  • Target See all Pkc beta 1 Antibodies
    Pkc beta 1 (Protein Kinase C, beta 1 (Pkc beta 1))
    Binding Specificity
    • 15
    • 3
    • 2
    • 1
    • 1
    pSer642
    Reactivity
    • 31
    • 29
    • 27
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat, Human, Mouse
    Host
    • 31
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 28
    • 9
    Polyclonal
    Conjugate
    • 12
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Pkc beta 1 antibody is conjugated to Cy3
    Application
    • 30
    • 14
    • 12
    • 12
    • 9
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human PKC beta 1 around the phosphorylation site of Ser642
    Isotype
    IgG
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    Discover our top product Pkc beta 1 Primary Antibody
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Pkc beta 1 (Protein Kinase C, beta 1 (Pkc beta 1))
    Alternative Name
    PKC beta 1 (Pkc beta 1 Products)
    Synonyms
    PKC-beta antibody, PKCB antibody, PRKCB1 antibody, PRKCB2 antibody, A130082F03Rik antibody, PKC-Beta antibody, Pkcb antibody, Prkcb1 antibody, Prkcb2 antibody, PKC antibody, PRKCB_tv2 antibody, protein kinase C beta antibody, protein kinase C, beta antibody, PRKCB antibody, Prkcb antibody
    Background

    Synonyms: Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1

    Background: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4.

    Gene ID
    5579
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