WEE1 antibody (pSer53)

Catalog No. ABIN711806

Product Details anti-WEE1 Antibody

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Binding Specificity: pSer53

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser53 [ED(p-S)AF]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: Wee1 (WEE1 Products)

Synonyms: WEE1A antibody, WEE1hu antibody, ATWEE1 antibody, F22D16.3 antibody, F22D16_3 antibody, WEE1 kinase homolog antibody, WEE1-KINASE antibody, WEE1 antibody, Xe-Wee1B antibody, XeWee1B antibody, wee1-b antibody, wee1a antibody, wee2-a antibody, xe-wee1a antibody, xwee1 antibody, wee1 antibody, wee1b antibody, wee1hu antibody, xwee2 antibody, GB19689 antibody, Wee1A antibody, fa20h03 antibody, wu:fa20h03 antibody, zgc:136514 antibody, WEE1 G2 checkpoint kinase antibody, Wee1 kinase antibody, WEE1-like kinase antibody, WEE1 G2 checkpoint kinase S homeolog antibody, wee1 homolog antibody, WEE1 homolog 2 antibody, wee1-like protein kinase antibody, WEE 1 homolog 1 (S. pombe) antibody, M phase inhibitor protein kinase Wee1 antibody, WEE1 antibody, Wee1 antibody, wee1.S antibody, wee1-a antibody, wee2 antibody, LOC551677 antibody, wee1 antibody

Background:

Synonyms: WEE1phospho S53, DKFZp686I18166, EC 2.7.10.2, FLJ16446, Wee 1, Wee1 homolog, WEE1 homolog S. pombe, Wee1 like protein kinase, Wee1 tyrosine kinase, Wee1+ homolog, Wee1+ S. pombe homolog, WEE1A, Wee1A kinase, WEE1hu, WEE1 tyrosine kinase isoform 1, MGC105683, WEE1_HUMAN.

Background: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Gene ID: 7465

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase