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SMAD3 antibody (pSer208) (Cy5.5)

SMAD3 Reactivity: Human, Mouse, Rat WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal Cy5.5
Catalog No. ABIN712231
  • Target See all SMAD3 Antibodies
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Binding Specificity
    • 38
    • 32
    • 31
    • 30
    • 25
    • 14
    • 12
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer208
    Reactivity
    • 252
    • 168
    • 150
    • 29
    • 12
    • 10
    • 9
    • 8
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 242
    • 23
    Rabbit
    Clonality
    • 230
    • 35
    Polyclonal
    Conjugate
    • 123
    • 15
    • 14
    • 13
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    This SMAD3 antibody is conjugated to Cy5.5
    Application
    • 202
    • 106
    • 88
    • 73
    • 54
    • 54
    • 52
    • 51
    • 27
    • 13
    • 11
    • 8
    • 5
    • 4
    • 2
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser208 [NL(p-S)PN]
    Isotype
    IgG
    Top Product
    Discover our top product SMAD3 Primary Antibody
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Alternative Name
    Smad3 (SMAD3 Products)
    Synonyms
    HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody
    Background

    Synonyms: SMAD3phospho S208, SMAD3phospho Ser208, p-SMAD3Ser208, hMAD 3, hSMAD3, HSPC193, JV15 2, JV152, MAD mothers against decapentaplegic Drosophila homolog 3, MAD3, MADH 3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD 3, SMAD, SMAD-3, SMAD3_HUMAN.

    Background: Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smads, and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

    Gene ID
    4088
    Pathways
    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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