SNCA antibody (AA 117-131)

Catalog No. ABIN7211663

Product Details anti-SNCA Antibody

Target: SNCA (Synuclein, alpha (SNCA))

Binding Specificity: AA 117-131

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNCA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: This antibody has been shown to react strongly with human brain-derived α-synuclein. The antibody also reacts with rat brain-derived α-synuclein but reactivity appears substantially less than with human.

Cross-Reactivity (Details): Does not cross-react with mouse α-synuclein.

Characteristics: NACP| Non-A β component of senile plaques,alpha-Synuclein polyclonal antibody

Purification: Purified by caprylic acid and ammonium sulphate precipitation.,Purified from rabbit serum. Vial contains an immunoglobulin preparation, partially purified by chromatography from whole serum, suspended in phosphate-buffered saline (pH 7.4) containing 0.01M sodium azide.

Immunogen: Synthetic peptide corresponding to [Cys] + aa 117-131 of human alpha-synuclein conjugated to KLH. The sequence is conserved in rat alpha-synuclein except for two substitutions, namely Asp121Ser and Asn122Ser.

Application Details

Application Notes: Western blot (1:1,000-1:20,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.,Western blot: The antibody has been characterized by single dimension SDS-PAGE using recombinant α- and β-synucleins as well as rat, mouse and human brain lysates with and without peptide blockade. Studies with the recombinant proteins demonstrated the absolute specificity of the antibody for α-synuclein. The antibody gives a strong band at a relative molecular weight of approximately 15 kDa with human brain lysate which is wholly abolished by preincubation with the immunizing peptide (Prod. No. BML-SP9340).Immunohistochemistry: The antibody has been tested on de-waxed tissue sections of human brain fixed in neutral buffered formaldehyde. A standard protocol should be followed.

Comment:

For Blocking peptide see Prod. No. BML-SP9340.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. In PBS, pH 7.4, containing 10 mM sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 °C.

Storage: -20 °C

Storage Comment: Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality 'antiserum-grade' plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: alpha-Synuclein (SNCA Products)

Synonyms: snca antibody, MGC64356 antibody, LOC619283 antibody, NACP antibody, alphaSYN antibody, PARK1 antibody, PARK4 antibody, PD1 antibody, synuclein alpha L homeolog antibody, alpha-synuclein antibody, synuclein alpha antibody, synuclein, alpha antibody, snca.L antibody, LOC619283 antibody, SNCA antibody, Snca antibody

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity