PSMB5 antibody (AA 213-263)

Catalog No. ABIN7453445

Product Details anti-PSMB5 Antibody

Target: PSMB5 (Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5))

Binding Specificity: AA 213-263

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-PSMB5 Antibody, Affinity Purified

Predicted Reactivity: Orangutan

Purification: Affinity Purified

Immunogen: Between AA 213 and 263

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PSMB5

Target: PSMB5 (Proteasome (Prosome, Macropain) Subunit, beta Type, 5 (PSMB5))

Alternative Name: PSMB5 (PSMB5 Products)

Synonyms: LMPX antibody, MB1 antibody, X antibody, etID309919.2 antibody, fb59c07 antibody, si:zc14a17.13 antibody, wu:fb59c07 antibody, x antibody, zgc:86820 antibody, proteasome subunit beta 5 antibody, proteasome (prosome, macropain) subunit, beta type 5 antibody, proteasome subunit beta 5 L homeolog antibody, proteasome (prosome, macropain) subunit, beta type, 5 antibody, PSMB5 antibody, psmb5 antibody, Psmb5 antibody, psmb5.L antibody

Background: Background: PSMB5 [proteasome (prosome, macropain) subunit, beta type, 5)] is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit) [taken from NCBI Entrez Gene (Gene ID: 5693)].

Gene ID: 5693

NCBI Accession: NP_002788

UniProt: P28074

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA