BAD antibody (AA 1-50)

Catalog No. ABIN7453524

The Rabbit Polyclonal anti-BAD antibody (ABIN7453524) specifically detects BAD in WB, IP and IHC (fp). The antibody is reactive with Human and Mouse samples.

Quick Overview for BAD antibody (AA 1-50) (ABIN7453524)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-BAD Antibody

Binding Specificity: AA 1-50

Purpose: Rabbit anti-BAD Antibody, Affinity Purified

Predicted Reactivity: Rat

Purification: Affinity Purified

Immunogen: between AA 1 and 50

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 10 μg/mg lysate

WB: Not recommended. ABIN7453524 has not performed satisfactorily when used for WB of BAD in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BAD.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background: Background: BAD (Bcl2 agonist of cell death) is a member of the Bcl-2 family. Bcl-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with Bcl-xL and Bcl-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein [taken from NCBI Entrez Gene (GeneID:572)].

Gene ID: 572

NCBI Accession: NP_004313

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events