PSMC1 antibody (AA 390-440)

Catalog No. ABIN7454963

Product Details anti-PSMC1 Antibody

Target: PSMC1 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 1 (PSMC1))

Binding Specificity: AA 390-440

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-PSMC1 Antibody, Affinity Purified

Predicted Reactivity: Rat

Purification: Affinity Purified

Immunogen: between AA 390 and 440

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PSMC1

Target: PSMC1 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 1 (PSMC1))

Alternative Name: PSMC1 (PSMC1 Products)

Synonyms: AI325227 antibody, P26s4 antibody, S4 antibody, P26S4 antibody, p56 antibody, s4 antibody, protease (prosome, macropain) 26S subunit, ATPase 1 antibody, proteasome 26S subunit, ATPase 1 antibody, Psmc1 antibody, PSMC1 antibody

Background: Background: Proteasome (prosome, macropain) 26S subunit, ATPase1 (PSMC1) is a subunit of the 26S proteasome, a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMC1 is one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. PSMC1 also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, PSMC1 interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder [taken from NCBI Entrez Gene (Gene ID: 5700)].

Gene ID: 5700

NCBI Accession: NP_002793

UniProt: P62191

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway