Raptor antibody (pSer792)

Catalog No. ABIN7464719

The Rabbit Polyclonal anti-Raptor antibody (ABIN7464719) specifically detects Raptor in WB. The antibody is reactive with Human samples.

Quick Overview for Raptor antibody (pSer792) (ABIN7464719)

Target: Raptor (RPTOR) (Regulatory Associated Protein of mTOR, Complex 1 (RPTOR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Raptor antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Raptor Antibody

Binding Specificity: pSer792

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser792 of human Raptor. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7(100 μM CCCP treatment for 2 hr)

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.21 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Raptor

Target: Raptor (RPTOR) (Regulatory Associated Protein of mTOR, Complex 1 (RPTOR))

Alternative Name: regulatory associated protein of MTOR complex 1

Background: Regulatory associated protein of MTOR complex 1 , KOG1 , Mip1,This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Molecular Weight: 149 kDa

Gene ID: 57521

UniProt: Q8N122

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Autophagy, BCR Signaling, Warburg Effect