ATP6V0A4 antibody (N-Term)
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- Target See all ATP6V0A4 Antibodies
- ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATP6V0A4 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Cross-Reactivity
- Mouse (Murine), Cow (Bovine)
- Cross-Reactivity (Details)
- Mouse (87 %), Bovine (82 %)
- Characteristics
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Rabbit polyclonal antibody to V-ATPase 116 kDa isoform a4 (ATPase, H+ transporting, lysosomal V0 subunit a4)
V-ATPase 116 kDa isoform a4 antibody [N1N3-2] - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human V-ATPase 116 kDa isoform a4. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product ATP6V0A4 Primary Antibody
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- Application Notes
- Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*
- Comment
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Positive Control: A431 , HeLa , HepG2 , Molt-4 , Raji
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- ATP6V0A4 (ATPase, H+ Transporting, Lysosomal V0 Subunit A4 (ATP6V0A4))
- Alternative Name
- V-ATPase 116 kDa Isoform a4 (ATP6V0A4 Products)
- Synonyms
- A4 antibody, ATP6N1B antibody, ATP6N2 antibody, RDRTA2 antibody, RTA1C antibody, RTADR antibody, STV1 antibody, VPH1 antibody, VPP2 antibody, Atp6n1b antibody, a4 antibody, ATPase H+ transporting V0 subunit a4 antibody, ATPase, H+ transporting, lysosomal V0 subunit A4 antibody, ATP6V0A4 antibody, Atp6v0a4 antibody
- Background
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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.
Cellular Localization: Apical cell membrane, Multi-pass membrane protein - Molecular Weight
- 96 kDa
- Gene ID
- 50617
- Pathways
- Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport
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