eIF4EBP1 antibody

Catalog No. ABIN7638530

The Rabbit Polyclonal anti-eIF4EBP1 antibody is suitable to detect eIF4EBP1 in samples from Rat. It has been validated for WB, IF and ICC.

Quick Overview for eIF4EBP1 antibody (ABIN7638530)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-eIF4EBP1 Antibody

Purpose: Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against EIF4EBP1. It has been selected for its ability to recognize EIF4EBP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPL481Ra01Recombinant Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500
Immunohistochemistry: 5-20 μg/mL,1:25-100
Immunocytochemistry: 5-20 μg/mL,1:25-100
Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: EIF4EBP1

Background: 4EBP1, BP-1, PHAS-I, Phosphorylated Heat And Acid-Stable Protein Regulated By Insulin 1

UniProt: Q62622

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling