GTPase NRas antibody (C-Term)

Catalog No. ABIN7825430

The Rabbit Polyclonal anti-GTPase NRas antibody (ABIN7825430) specifically detects GTPase NRas in WB, IHC and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for GTPase NRas antibody (C-Term) (ABIN7825430)

Target: GTPase NRas (NRAS)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-GTPase NRas Antibody

Binding Specificity: C-Term

Purpose: Anti-NRAS Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human NRAS shares 100% amino acid (aa) sequence identity with both mouse,rat NRAS.

Characteristics: Anti-NRAS Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NRAS. Human NRAS shares 100% amino acid (aa) sequence identity with both mouse and rat NRAS.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background:

Background: This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.

Gene Full Name: NRAS proto-oncogene, GTPase

Molecular Weight: 21 kDa

Gene ID: 4893

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling