PDK4 antibody (AA 85-129)

Catalog No. ABIN7876971

The Rabbit Polyclonal anti-PDK4 antibody is suitable to detect PDK4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, IHC (p) and FACS.

Quick Overview for PDK4 antibody (AA 85-129) (ABIN7876971)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-PDK4 Antibody

Binding Specificity: AA 85-129

Purpose: Pyruvate dehydrogenase kinase 4 Antibody / PDK4

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (amino acids V85-N129) was used as the immunogen for the Pyruvate dehydrogenase kinase 4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Pyruvate dehydrogenase kinase 4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Pyruvate dehydrogenase kinase 4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Alternative Name: Pyruvate dehydrogenase kinase 4

Background: Pyruvate dehydrogenase lipoamide kinase isozyme 4, mitochondrial (PDK4) is an enzyme that in humans is encoded by the PDK4 gene. This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.

UniProt: Q16654

Pathways: PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect