GTPase NRas antibody

Catalog No. ABIN7938428

The Rabbit Polyclonal anti-GTPase NRas antibody is suitable to detect GTPase NRas in samples from Human. It has been validated for WB.

Quick Overview for GTPase NRas antibody (ABIN7938428)

Target: GTPase NRas (NRAS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTPase NRas antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GTPase NRas Antibody

Purpose: NRAS antibody (pAb)

Purification: Protein A Chromatography

Immunogen: This information is proprietary to Active Motif and/or its suppliers.

Isotype: IgG1

Application Details

Application Notes: *WB: 1 - 4 μg/mL dilution *For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05 % Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

Target Details for GTPase NRas

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background:

Background_Plain: Oncogenic mutations in Ras genes have been found in about 30 % of human cancers. This family of genes is involved in cell signaling pathways that control cell growth and death. The human Ras gene family consists of three identified members H-, K- and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons. Human H- and K-ras are the homologues of v-H- and v-K-ras, sequences. Under normal circumstances the Ras-GTPase activity gradually converts GTP to GDP, but in oncogenic mutations Ras locks the protein into the active GTP conformation. This leads to a constant Ras gene and uncontrollable cell growth. Through signal transduction, NRAS is involved in regulating cells division. The information is received from outside the cell to the cell's nucleus to instruct the cell to proliferate, to mature or to differentiate. Due to these intracellular relationship Ras antibodies have emerged as a valuable tool for target validation studies for cancer.

Aliases: Neuroblastoma RAS Viral Oncogene Homolog, nras, Nras, NRAS

Molecular Weight: 21 kDa

NCBI Accession: NP_002515

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling