FOXO3 antibody (acLys569)

Catalog No. ABIN8121505

The Rabbit Polyclonal anti-FOXO3 antibody (ABIN8121505) specifically detects FOXO3 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FOXO3 antibody (acLys569) (ABIN8121505)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FOXO3 Antibody

Binding Specificity: acLys569

Purpose: FoxO3A (Acetyl Lys569) Antibody

Specificity: This antibody detects endogenous levels of Human,Mouse,Rat FoxO3A (Acetyl Lys569)

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human FoxO3A (Acetyl Lys569)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FoxO3A

Background:

Synonyms: Forkhead box protein O3 (AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1)

Background: disease:A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6,11)(q21,q23) with MLL/HRX.,function:Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.,PTM:In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Translocates to the nucleus upon oxidative stress and in the absence of survival factors.,subunit:Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation.,tissue specificity:Ubiquitous.,

Gene Name: FOXO3 FKHRL1 FOXO3A

Protein Name: FoxO3A (Acetyl Lys569)

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis