CDK5 antibody (pTyr15) (HRP)

Catalog No. ABIN879032

Product Details anti-CDK5 Antibody (HRP)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Binding Specificity: pTyr15

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDK5 around the phosphorylation site of Tyr15

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5 (CDK5 Products)

Synonyms: PSSALRE antibody, AW048668 antibody, Crk6 antibody, CDK5 antibody, CG8203 antibody, DmCdk5 antibody, Dmel\\CG8203 antibody, cdk5 antibody, zgc:101604 antibody, cyclin dependent kinase 5 antibody, cyclin-dependent kinase 5 antibody, Cyclin-dependent kinase 5 antibody, cyclin-dependent kinase 5 L homeolog antibody, Cyclin-dependent-like kinase 5 antibody, CDK5 antibody, Cdk5 antibody, cdk5 antibody, cdk5.L antibody, cdk-5 antibody

Background:

Synonyms: Cdk5 phospho Y15, p-Cdk5Y15, Cdk 5, Cell division protein kinase 5, Crk6, Cyclin dependent kinase 5, Protein kinase CDK5 splicing, PSSALRE, Serine threonine protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit, CDK5_HUMAN.

Background: CDK5 (Cyclin Dependent Kinase 5) is serine/threonine kinase involved in synaptic regulation and neuronal development, phosphorylates synaptic protein Pctaire1, regulates acetylcholine receptor expression. CDK5 is a member of the cyclindependent kinase family of serine/threonine kinases. It is present in numerous mammalian tissues including kidney, testes, and ovary. Its activity is detected almost exclusively in brain extracts. Neuronal and muscle cells contain the highest amount of this protein. Similar to other Cdks, monomeric Cdk5 displays no enzymatic activity, but Cdk5 is not activated by cyclins. Instead, the p35 protein, which is expressed solely in the brain, activates Cdk5. Cdk5 interacts with D1 and D3 type G1 cyclins and can phosphorylate histone H1, TAU, MAP2 and NF-H and NF-M. Cdk5 activity is involved in terminal differentiation of neurons and muscle cells.

Gene ID: 1020

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis