CDC25A antibody (Middle Region)

Catalog No. ABIN926907

Product Details anti-CDC25A Antibody

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Frog, Zebrafish (Brachydanio rerio), Chicken

Purification: Purified

Immunogen: CDC25 A antibody was raised in rabbit using the middle region of CDC25 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CDC25A Blocking Peptide, catalog no. 33R-5934, is also available for use as a blocking control in assays to test for specificity of this CDC25A antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: CDC25A (CDC25A Products)

Synonyms: cdc25a2 antibody, cdc25A antibody, sb:cb317 antibody, CDC25A2 antibody, D9Ertd393e antibody, cell division cycle 25A antibody, cell division cycle 25B antibody, cell division cycle 25A S homeolog antibody, CDC25A antibody, cdc25a antibody, cdc25b antibody, Cdc25a antibody, cdc25a.S antibody

Background: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. Synonyms: Polyclonal CDC25A antibody, Anti-CDC25A antibody, cell division cycle 25 homolog A, S. pombe antibody, CDC25A2 antibody.

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase