BAD antibody (C-Term)

Catalog No. ABIN927291

The Rabbit Polyclonal anti-BAD antibody (ABIN927291) specifically detects BAD in WB. The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for BAD antibody (C-Term) (ABIN927291)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAD Antibody

Binding Specificity: C-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine)

Purification: Purified

Immunogen: BAD antibody was raised in rabbit using the C terminal of BAD as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

BAD Blocking Peptide, (ABIN5612269), is also available for use as a blocking control in assays to test for specificity of this BAD antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background: BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. Synonyms: Polyclonal BAD antibody, Anti-BAD antibody, BCL2-antagonist of cell death antibody, BBC2 antibody, BCL2L8 antibody.

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events