NCF4 antibody (C-Term)

Catalog No. ABIN929086

Product Details anti-NCF4 Antibody

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF4 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Purified

Immunogen: NCF4 antibody was raised in rabbit using the C terminal of NCF4 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

NCF4 Blocking Peptide, catalog no. 33R-7359, is also available for use as a blocking control in assays to test for specificity of this NCF4 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for NCF4

Target: NCF4 (Neutrophil Cytosolic Factor 4, 40kDa (NCF4))

Alternative Name: NCF4 (NCF4 Products)

Synonyms: p40phox antibody, AI451400 antibody, NCF antibody, P40PHOX antibody, SH3PXD4 antibody, neutrophil cytosolic factor 4 antibody, ncf4 antibody, Ncf4 antibody, NCF4 antibody

Background: NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. Synonyms: Polyclonal NCF4 antibody, Anti-NCF4 antibody, neutrophil cytosolic factor 4, 40kDa antibody, MGC3810 antibody, NCF antibody, P40PHOX antibody, SH3PXD4 antibody.