HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (C-Term) antibody

Details for Product No. ABIN94383
Antigen
Epitope
C-Term
29
11
8
8
7
7
4
4
3
3
3
2
2
1
1
1
1
1
Reactivity
Human
323
9
8
5
1
1
1
1
Host
Mouse
219
82
26
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
43
41
25
18
11
5
2
2
2
2
2
2
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
184
170
170
110
63
47
35
33
22
18
14
13
8
3
2
2
2
1
1
Options
Immunogen C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin.
Clone 2A12
Isotype IgG1
Specificity The antibody 2A12 was generated to a peptide corresponding to C-intron 4-encoded sequence. This antibody does not crossreact with the full-length HLA-G1 isoform and thus allow to distinguish between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name HLA-G (HLAG Antibody Abstract)
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) (C-Term) antibody (ABIN94383) Surface staining of HLA-G (on transfected cells) with anti-HLA-G (2A12) purified, GAM...
Product cited in: Poláková, Železníková, Russ: "HLA-G5 in the blood of leukemia patients and healthy individuals." in: Leukemia research, Vol. 37, Issue 2, pp. 139-45, 2013 (PubMed).

Larsen, Bzorek, Pass, Larsen, Nielsen, Svendsen, Lindhard, Hviid: "Human leukocyte antigen-G in the male reproductive system and in seminal plasma." in: Molecular human reproduction, Vol. 17, Issue 12, pp. 727-38, 2011 (PubMed).

White, Loisel, McConville, Stern, Tu, Marroquin, Noth, Ober: "Levels of soluble human leukocyte antigen-G are increased in asthmatic airways." in: The European respiratory journal, Vol. 35, Issue 4, pp. 925-7, 2010 (PubMed).

LeMaoult, Le Discorde, Rouas-Freiss, Moreau, Menier, McCluskey, Carosella: "Biology and functions of human leukocyte antigen-G in health and sickness." in: Tissue antigens, Vol. 62, Issue 4, pp. 273-84, 2003 (PubMed).