STAT1 antibody (pSer727)

Catalog No. ABIN94473

Cited in 2+ publications. The Mouse Monoclonal anti-STAT1 antibody (Clone PSM1) (ABIN94473) specifically detects STAT1 in WB and IP. The antibody is reactive with Human samples.

Quick Overview for STAT1 antibody (pSer727) (ABIN94473)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: PSM1

Product Details anti-STAT1 Antibody

Binding Specificity: AA 721-733, pSer727

Purpose: Anti-Hu STAT1 (pS727) Purified

Cross-Reactivity (Details): Other not tested, Human

Characteristics: The antibody PSM1 recognizes transcriptional factor STAT1 (91 kDa) activated by phosphorylation at Ser727 (intracellular antigen).

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: STAT1 peptide sequence 721-733 (Ser727 phosphorylated).

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-STAT1 antibody (ABIN94473)

Kovarik, Boudny, Kocak, Lauerova, Fait, Vagundova: "Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation." in: International journal of molecular medicine, Vol. 12, Issue 3, pp. 335-40, (2003) (PubMed).

Boudný, Kocák, Lauerová, Kovarík: "Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients." in: Folia biologica, Vol. 49, Issue 4, pp. 142-6, (2003) (PubMed).

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1

Background: Signal transducer and activator of transcription 1,STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.,CANDF7, IMD31, ISGF3

Gene ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events