eIF4EBP1 antibody

Catalog No. ABIN965483

There are 2+ publications for this product available. The Monoclonal anti-eIF4EBP1 antibody is suitable to detect eIF4EBP1 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for eIF4EBP1 antibody (ABIN965483)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-eIF4EBP1 Antibody

Specificity: Ni-NTA purified truncated recombinant 4EBP1 expressed in E. Coli strain BL21 (DE3).

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1: 500- 1: 1,000.
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-eIF4EBP1 antibody (ABIN965483)

Fadden, Haystead, Lawrence: "Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10240-7, (1997) (PubMed).

Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, (1994) (PubMed).

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: 4E-BP1

Background: 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Gene ID: 1978

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling