AKT3 antibody

Catalog No. ABIN965532

Cited in 2+ publications. The Monoclonal anti-AKT3 antibody (ABIN965532) specifically detects AKT3 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for AKT3 antibody (ABIN965532)

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This AKT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AKT3 Antibody

Specificity: Ni-NTA purified truncated recombinant Akt3 expressed in E. Coli strain BL21 (DE3)

Purification: Crude ascites.

Application Details

Application Notes: Western Blot: 1: 500- 1: 2000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

References for anti-AKT3 antibody (ABIN965532)

Easton, Cho, Roovers, Shineman, Mizrahi, Forman, Lee, Szabolcs, de Jong, Oltersdorf, Ludwig, Efstratiadis, Birnbaum: "Role for Akt3/protein kinase Bgamma in attainment of normal brain size." in: Molecular and cellular biology, Vol. 25, Issue 5, pp. 1869-78, (2005) (PubMed).

Stahl, Sharma, Cheung, Zimmerman, Cheng, Bosenberg, Kester, Sandirasegarane, Robertson: "Deregulated Akt3 activity promotes development of malignant melanoma." in: Cancer research, Vol. 64, Issue 19, pp. 7002-10, (2004) (PubMed).

Target Details for AKT3

Target: AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))

Alternative Name: Akt3

Background: Akt3 (also designated protein kinase B gamma or vakt murine thymoma viral oncogene homolog 3),with 479-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt2. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake.Akt3 is not required for the maintenance of normal carbohydrate metabolism but is essential for the attainment of normal organ size. Identifying Akt3 as a selective target in melanoma cells also provides new therapeutic opportunities for patients in the advanced stages of this disease.

Gene ID: 10000

Pathways: PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling