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Polyclonal Antibodies

Polyclonal antibodies are antibodies that are secreted by different B cell lineages within the host (whereas monoclonal antibodies come from a single cell lineage). They are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Choose between properties like host, clone and antigen to get the antibody you need. Our staff supports you finding the right product via phone, live chat or email.

If you want to use an unconjugated primary, don't forget the corresponding secondary antibody!

Important Targets

beta Actin Antibodies

beta Amyloid Antibodies

Caspase 8 Antibodies

beta Catenin Antibodies

COL1 Antibodies

NFKB1 Antibodies

c-FOS Antibodies

TNF alpha Antibodies

WNT2 Antibodies

All Polyclonal Antibodies

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601,847 results

RFP antibody

RFP Reactivity: Discosoma WB, ELISA, IF, IHC, IP, FACS, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

356 references

Independent Validation

1 validation

Catalog No. ABIN129578

Independently Validated

beta Actin antibody (AA 1-50)

ACTB Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Rat lung lysate 30ug, probed (ABIN724340) at 1:200 overnight in 4 °C.

Image kindly submitted by Piotr Mamczur from Wroclaw University.

Human HeLa cell lysates probed with Rabbit Anti-beta Actin Polyclonal Antibody, Unconjugated .

Image kindly submitted by Piotr Mamczur from Wroclaw University.

Hepatic overexpression of HNF4α in rats decreased cholesterol levels in both plasma and liver.

Expression pattern of hepatic cholesterol-regulatory genes under HNF4α overexpression in rat liver.

HNF4α-upregulated SR-BI depends on PPARγ.

High-temperature cultivation of an rhIL-10 expression strain impairs the maturation of G3-pro-rhIL10.

High-temperature cultivation of an rhIL-10 expression strain impairs ER folding capacity.

High-temperature cultivation of an rhIL-10 expression strain impairs ER folding capacity.

(a) Expression of viral N (Nucleoprotein) and G (Glycoprotein) genes mRNA in zebrafish embryonic fibroblast cell line (ZF4) with time after snakehead fish vesiculovirus (SHVV) infection.

(a) Expression of viral N (Nucleoprotein) and G (Glycoprotein) genes mRNA in zebrafish embryonic fibroblast cell line (ZF4) with time after snakehead fish vesiculovirus (SHVV) infection.

Expression of Mx and interferon (IFN) genes in ZF4 with time after SHVV infection.

Expression of Retinoic acid-inducible gene I (RIG-I), Melanoma differentiation-associated gene 5 (MDA5), LGP2T, and LGP2a genes in ZF4 with time after SHVV infection.

(a) The expression of DrLGP2a, DrLGP2b proteins during the infection with SHVV.

Pro-Gly, but not Pro plus Gly (Pro+Gly), promoted the expression and secretion of IGF-1 in the HepG2 cells.

Knockdown of PepT1 eliminated the increase of IGF-1 expression induced by Pro-Gly in the HepG2 cells.

Pro-Gly activated JAK2/STAT5 signaling pathway in a PepT1-dependent manner.

Pro-Gly activated JAK2/STAT5 signaling pathway in a PepT1-dependent manner.

(a) The expression of DrLGP2a, DrLGP2b proteins during the infection with SHVV.

Inhibition of JAK2/STAT5 signaling pathway blocked the promotive effect of Pro-Gly on IGF-1 expression and secretion in the HepG2 cells.

Acute or chronic injection of Pro-Gly, but not Pro plus Gly (Pro+Gly), stimulated IGF-1 expression and secretion in mice.

Acute or chronic injection of Pro-Gly, but not Pro plus Gly (Pro+Gly), stimulated IGF-1 expression and secretion in mice.

Injection of JAK2/STAT5 inhibitor abolished the promotive effect of Pro-Gly on IGF-1 expression and secretion in mice.

Protein expression of MTA1 by Western Blot analysis.

Effects of stable YAP1 silencing in BGC-823 cells and stable YAP1 overexpressing in GES-1 cells on migration and invasion abilities in vitroThe wound healing assay was used to evaluate the migration properties of BGC-823 GC cells (A, G) and GES-1 cells (B, H).

Effects of JQ1 treatment on cell proliferation, migration and invasion in ccRCC cell lines(A) Cell proliferation was determined by XTT assays (*P < 0.

JQ1 treatment inhibited MYC expression in ccRCC cells(A) The expression level of MYC mRNA was significantly upregulated in ccRCC tissues compared with that in normal kidney tissues according to TCGA data (P < 0.

Protein levels of NF-κB and NLRP3/Caspase-1 pathway in CIA rats.

Clinical significance of BRD4 expression in ccRCC according to TCGA data(A) Overall survival (OS) was significantly shortened in patients with high BRD4 expression (top third) compared with that in patients with medium and low BRD4 expression (P = 0.

The effect of LBP on the expression of GFAP, VEGF, and PEDF in the retinal tissue.

Relative expression of AANAT, HIOMT, MT1, and MT2 in COCs of follicles of different sizes.

Effect of E2 and ER antagonist ICI182780 on melatonin production and the expression of AANAT, HIOMT, MT1, and MT2 mRNA and protein in sheep COCs.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

Arginine inhibits HIF-1α to reduce LDHA expression after cerebral I/R injury.

NLRP3 inflammasome activation was involved in P2Y14R deficiency.

Upregulated cAMP suppressed acute gouty arthritis in vivo.

Pyroptosis stress was invalidated once cAMP increased by Forskolin in vitro.

Decreased cAMP exaggerated acute gouty arthritis in P2Y14R-KO rats.

43 images

272 references

Catalog No. ABIN724340

COL1A2/COL1A1 antibody

Rockland 600-401-103-0.1 Reactivity: Human, Cow, Mouse, Pig, Rat ELISA, WB, DB, FLISA, FACS, FM, IHC, IP Host: Rabbit Polyclonal unconjugated

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Immunohistochemistry validation image for anti-COL1A2/COL1A1 antibody (ABIN5596819)

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Immunofluorescence staining of extracellular and intracellular type I collagen.

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

The testing of multi-differentiation of rabbit TSCs in vivo.

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

SMT022357 improves the pathology in the diaphragm.

Histological analysis of muscle and tendon monoculture tissue model differentiation.

CYD0682 suppresses endogenous ECM protein expression.

Collagen type I expression in the IVD tissue.

Both serum-free and serum-containing fibrocytes express both haematopoietic and stromal cell markers.

The augmented participation from infiltrating monocytes into myeloid-dependent fibrosis in the aging B6J heart.

Immunoperoxidase stain for collagen type I (a–c), collagen type III (d–f) and collagen type VI (g–i).

At least two grid per tissue biopsies were prepared.

Collagen Far Western blot of B.

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

Oridonin suppresses α-SMA and ECM protein expression.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

p21 knockout alleviates liver fibrosis.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

Western blot of Human Collagen Type I.

Expression of collagen by 5 day cultured spleen cells.

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Immunofluorescence staining of extracellular and intracellular type I collagen.

46 images

62 references

Independent Validation

2 validations

Catalog No. ABIN5596819

Independently Validated

GFP antibody

GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Goat Polyclonal unconjugated

Western Blot of Goat anti-GFP antibody.

MSCs are traced in special organized regions.

Immunofluorescence Microscopy of GFP-GOAT-Antibody.

Western Blot of Rabbit anti-GFP antibody.

Immunofluorescence Microscopy of GFP-GOAT-Antibody.

Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody.

Western Blot of Goat anti-GFP antibody with lysates.

Western Blot of Goat anti-GFP Antibody with Serums.

Tyrosine phosphorylation of HIPK mutants.

Tyrosine phosphorylation of HIPK mutants.

Tyrosine phosphorylation of HIPK mutants.

Tyrosine phosphorylation of HIPK mutants.

Maximal activity of HIPKs depends on the activation loop tyrosine.

Subcellular distribution of HIPK mutants.

15 images

178 references

Catalog No. ABIN100085

GAPDH antibody

GAPDH Reactivity: Human WB, IF, IP, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in mouse testis by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH, antibody (green) at 1:500 dilution.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IP Image Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody, Western blot analysis was performed using GAPDH antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Immunohistochemical analysis of human salivary gland cancer, using GAPDH, antibody at 1:500 dilution.

WB Image GAPDH antibody detects GAPDH protein by western blot analysis.

IHC-P Image GAPDH antibody detects GAPDH protein at cytoplasm in rat heart by immunohistochemical analysis.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Immunoblotting of LEG-1 from the treated fibroblasts and the measurement of ROS within the treated fibroblasts.

Effects of quercetin on the inflammatory response in H2O2-treated H9C2 cells.

Effects of quercetin on cell apoptosis in H2O2-treated H9C2 cells.

Comprehensive immunofluorescence images and immunoblotting analysis of the differentially expressed proteins identified by MALDI-TOF MS.

Gene trap screen for mitochondrial phenotypes identifies Sucla2 mutant allele.

Sucla2 mutant embryos display growth deficiency and placental mtDNA depletion.

Sucla2-deficient embryo tissues exhibit progressive, functionally significant mtDNA depletion and elevated MMA.

CM extract induces cell apoptosis.

Effects of quercetin on doxorubicin-induced changes of cell viability, cell apoptosis and cell morphology in H9C2 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

RAGE expression in human peripheral nerve fibers.

Arecoline induces the transdifferentiation of buccal mucosal fibroblasts (BMFs) into myofibroblasts.

Arecoline-induced α-smooth muscle actin (α-SMA) expression is mediated by the direct binding of zinc finger E-box binding homeobox 1 (ZEB1) to the α-SMA promoter.

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction.

Long-term exposure of buccal mucosal fibroblasts (BMFs) to arecoline induces fibrogenic gene expression and collagen contraction.

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R).

Arecoline-induced zinc finger E-box binding homeobox 1 (ZEB1) expression is independent of reactive oxygen species, but is mediated by the activation of insulin-like growth factor receptor-1 (IGF-1R).

Regulation of NED by REST in LNCaP cells.

Regulation of NED by REST in LNCaP cells.

Regulation of NED by REST in LNCaP cells.

IL-6 induces NED in LNCaP cells and this is concomitant with increased autophagy.

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of beclin1 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions.

Knockdown of Atg5 suppresses IL-6 induced NED under androgen deprivation conditions.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

IL-6 treatment inhibits mTOR via the activation of AMPK pathway.

Modulation of curcumin in oxidative stress-regulating genes.

Arecoline decreased membrane expression of ZO-1 in confluent TM4 cells.

Arecoline activates ERK1/2 MAPKs and increases TNF-alpha production.

Activation of ERK1/2 MAPKs is important for TNF-alpha induction and ZO-1 protein redistribution by arecoline in TM4 cells.

DZNep-mediated sensitization to TRAIL correlates with enhanced activation of caspase signaling and involvement of the mitochondrial apoptosis pathway.

Early and accelerated processing of caspase-8 with DZNep treatment in lymphoma cell lines.

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels.

DZNep-mediated enhanced apoptosis correlates with reduced cFLIP expression levels.

Effects of different proteins on post-treatment lipolysis markers.

Creation and characterization of Supt4h knockout mice.

Role of STAT3 expressionA.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

Western blot analysis of FOXM1 and potential target expression in GEP-NEN cell linesAll cell lines including the non-neuroendocrine HT-29 and the FOXM1 overexpressing U2OS control cell lines expressed FOXM1 A.

Dicer inactivation in mutant mice.

The expression of BH3-only protein, pro-apoptotic proteins and anti-apoptotic proteins in LNCaP or PC3 cells after treatment with paclitaxel.

The effect of ABT-199, ABT-263 or ABT-263 in combination with paclitaxel on LNCaP or PC3 cells.

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP.

NS5 with mutation on residue 19 (M19A) showed reduced binding ability with MTP.

Diverse fuels promote TNKS autoPARsylation and turnover in INS-1 cells.

Promoter hypermethylation of sGCβ1 in MCF-7 and sGCα1 in MDA-MB-231 cells.

Over-expression of sGCα1 and sGCβ1 in MDA-MB-231 cells affected cGMP production, proliferation, survival, and cell cycle.

Over-expression of sGC induced apoptosis and cell cycle arrest.

HYS-32 modulates GSK3β phosphorylation.

GSK3β inhibitor SB415286 inhibits the HYS-32-induced phosphorylation of GSK3β-pY216 and attenuates the HYS-32-induced microtubule catastrophes.

Caspase-dependent apoptotic pathways were seen in LNCaP, but not in PC3 cells, in response to G2/M arrest.

LY294002 inhibits the HYS-32-induced phosphorylation of GSK3β-pS9 and GSK3β-pY216.

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL.

Overexpression of miR-29a in the murine model resulted in downregulation of fibrosis and HDAC4 in the liver of mice after BDL.

Effects of TLCA on HDAC4 expression in cultured primary HSCs.

Overexpression of miR-29a decreased HDAC4 expression in primary HSCs.

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs.

Overexpression of miR-29a increased histone H3 at lysine 9 (H3K9Ac) and decreased Smad3 expression in HSCs.

Western blot analysis shows pErk reduction through selumetinib treatment and by removal of stromal support.

Phospho-flow measures long-term inhibition by selumetinib of pErk generated in BCP-ALL cells by extracellular serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Diagnosis ALL samples differ in ability to activate Erk1/2 upon serum stimulation.

Mek pathway inhibition in combination-treated BCP-ALL cells by phospho-flow and Western blot.

Demyelination/remyelination and decreased P0 expression in dKO mice.

Loss of neurofascins and Caspr in dKO mice.

P0 interacts with neurofascins.

ETO inhibits ACT cell growth.

Inhibition of ERK1/2 inhibits ETO-induced centrosome amplification.

Chloroquine inhibits DNA damage-induced centrosome amplification.

Chloroquine inhibits ETO-activated CDK2 and ERK1/2 in adrenocortical Y1 tumor cells.

HSPs positively regulate IE2 transaction activity.

Comparison of the mRNA levels of Smad7 (a) and protein levels of Smad3 (b) expressions in the liver of WT and miR-29Tg mice following bile duct ligation (BDL).

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

Overexpression of miR-29a in the murine model caused the upregulation of β-catenin (a,b), and Dkk1 (d) as well as the downregulation of phospho-β-catenin (c) and GSK3β (e) in the liver of mice following bile duct ligation.

SFN suppressed HCV protein expression and RNA replication.

SFN-mediated HO-1 induction in HCV replicon cells.

SFN inhibited HCV replication through HO-1 dependent pathway.

SFN inhibited HCV replication through HO-1 dependent pathway.

SFN induced bilirubin production in HCV replicon cells.

SFN inhibited HCV replication by upregulating Nrf2 expression.

SFN-mediated Nrf2/HO-1 activation was PI3K dependent.

SFN-mediated Nrf2/HO-1 activation was PI3K dependent.

Hypoxia induces NED of LNCaP cells concomitant with down-regulation REST protein levels but not REST mRNA(A) LNCaP cells were treated with hypoxia (2 % O2) for 3 days.

Inhibition of neurite elongation by REST overexpression(A) LNCaP-TR-REST cells were treated with 0.

AMPK/mTOR pathway is activated by hypoxia treatment and REST knockdown(A) LNCaP cells were treated with normoxia (N) or hypoxia (H) (2 % O2) for 3 days (left panel).

Hypoxia-induced autophagy activation of LNCaP cells(A) LNCaP-eGFP-LC3 cells were treated with hypoxia (2 % O2) for 3 days.

Chemical inhibition of autophagy flux by chloroquine (CQ) suppresses hypoxia-induced NED in LNCaP cells(A) LNCaP cells were treated with hypoxia (2 % O2) in the absence or presence of 50 μM CQ for 3 days.

Knockdown of beclin 1 suppresses hypoxia-induced NED(A) LNCaP-TR-shBeclin1 cells were treated with 1 μg/mL Dox to induce beclin 1 knockdown for 4 days under normoxia and hypoxia (2 % O2).

Disruption of autophagy by treatment with CiQ derivativesA.

Disruption of autophagy by treatment with CiQ derivativesA.

Induction of apoptosis by treatment with CiQ derivativesA.

Induction of lysosome dysfunction by treatment with CiQ derivativesA and B.

Differential expression of CDK1 pTyr15 in colorectal cancer cells and tissues.

Senataxin is SUMOylated at the XY chromosomes in spermatocytes.

120 images

105 references

Catalog No. ABIN2857072

Caspase 8 antibody (AA 411-482)

CASP8 Reactivity: Human, Mouse, Rat, Monkey, Pig, Sheep WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 images

15 references

Independent Validation

1 validation

Catalog No. ABIN724205

Independently Validated

TNF alpha antibody (AA 181-235)

TNF alpha Reactivity: Human, Mouse, Rat, Rabbit, Horse WB, ELISA, IHC (p), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Histology and immunofluorescence in rat corneal sections.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

11 images

21 references

Independent Validation

1 validation

Catalog No. ABIN677318

Independently Validated

E-cadherin antibody (AA 401-500)

CDH1 Reactivity: Human, Mouse, Rat WB, FACS, ELISA, ICC, IHC (p), IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847)

Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.

MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

Image provided by One World Lab validation program.

Image provided by Independent Validation badge 29768.

Image provided by One World Lab validation program.

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

14 images

14 references

Independent Validation

1 validation

Catalog No. ABIN1387847

Independently Validated

Dendra 2 antibody

Reactivity: Coral WB, FACS, ICC Host: Rabbit Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Western blotting analysis of Dendra2/GPI fusion protein using rabbit polyclonal antibody PAb (836) on lysates of HEK293T/17 cells transfected with Dendra2/GPI construct, reducing and non-reducing conditions.

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

Flow cytometry surface staining pattern of HEK293T/17 cells transfected with Dendra2/GPI construct stained using anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC).

Separation of HEK293T/17 cells transfected with Dendra2/GPI construct stained anti-Dendra2 purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells transfected with Dendra2/GPI construct unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/Dendra2/GPI cell suspension.

8 images

16 references

Independent Validation

1 validation

Catalog No. ABIN361314

Independently Validated

CD63 antibody (AA 120-175)

CD63 Reactivity: Human, Mouse, Rat, Monkey, Dog FACS, WB, IHC, IF Host: Goat Polyclonal unconjugated

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting validation image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Anti-CD63 Ab at 1/1,000 dilution; Iysate at 50 µg per lane; Rabbit polyclonal to goat lgG (HRP) at 1/10,000 dilution

Immunofluorescence — anti-CD63 Ab in Hepa1-6 cells at 1/50 dilution; cells were fixed with 4% of PFA;

Immuno-localization of CD63 in the hen reproductive trac

Representative immunogold labeling (using TEM) images of CSF-derived EVs from control and prenatally cocaine-exposed male and female monkeys are shown.

Flow cytometry analysis of membrane CD63 level in PMN of control and SCK cows.

Expression of exosomes in bronchoalveolar lavage in Rat BAL fluid Source: PMID36035371

Expression of exosomes in Rat lung tissue Source: PMID36035371

Expression of exosomes in Rat Brain Tissue Source: PMID36035371

Immunofluorescence (IF) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Western Blotting (WB) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

Immunohistochemistry (IHC) image for anti-CD63 (CD63) (AA 120-175) antibody (ABIN1440014)

18 images

10 references

Independent Validation

3 validations

Catalog No. ABIN1440014

Independently Validated

SOX2 antibody

KO Validated SOX2 Reactivity: Human WB, IF, FACS, ICC, IHC (p), IP, IHC (fro), CUT&RUN Host: Rabbit Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

Cleavage Under Targets and Release Using Nuclease validation image for anti-SRY (Sex Determining Region Y)-Box 2 (SOX2) antibody (ABIN2855074)

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2, antibody at 1:100 dilution.

IP Image Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] , or SOX2 antibody [GT1876], Western blot analysis was performed using SOX2 antibody [N1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2, antibody at 1:160 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2, antibody at 1:80 dilution.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.

IHC-P Image SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.

FACS Image Flow cytometry on human embryonic stem cells, staining with SOX2 ,antibody at 1:100 dilution(purple) or rabbit IgG (black).

WB Image SOX2 antibody detects SOX2 protein by western blot analysis.

Western blot analysis of CD44, octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2), SOX9, transketolase (TKT), hypoxia-inducible factor 1 alpha (HIF-α) and phosphoglycerate kinase 1 (PGK-1) in CSC-like and non-CSC UM1 cells (A).

KD of Cops2, but not any other CSN subunits, reduces the expression of Nanog and Oct4.

In vitro characterizations of hc-iPSCs.

S1PR1 activation enhanced NSCs proliferation and neuronal differentiation in hippocampal dentate gyrus (DG) after TBI.

S1PR1-induced NSCs proliferation and neuronal differentiation in TBI rats were affected by MEK/Erk activity.

17 images

11 references

Independent Validation

1 validation

Catalog No. ABIN2855074

Independently Validated

Iba1 antibody (C-Term)

IBA1 Reactivity: Human WB, IHC, ICC, IF, IHC (p), FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

Immunofluorescence validation image for anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody (ABIN2857032)

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 references

Independent Validation

1 validation

Catalog No. ABIN2857032

Independently Validated

Caspase 3 antibody (AA 60-100)

CASP3 Reactivity: Human, Mouse, Rat, Cow, Goat WB, IF (cc) Host: Rabbit Polyclonal unconjugated

Rat lung lysate 30ug, probed at 1:200 overnight in 4˚C.

Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (ABIN724625) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C and DAPI staining

Human HepG2 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody

Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Neuronal apoptosis in acNTS of CRS mice.

Erianin induced mitochondrial apoptosis in liver cancer cells.

7 images

86 references

Catalog No. ABIN724625

Vimentin antibody (AA 371-466)

VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

Mouse spleen lysates probed with Anti-Vimentin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (ABIN672786) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated (ABIN672786) at 1:300, followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program.

Image submitted by One World Lab validation program.

20 μg proteins of cell lysates from U138, HeLa and A431 were separated by SDS-PAGE and transferred to PVDF membrane for western blotting analysis antibodies against protein VIM at dilution of 1:1000 with exposure time of 10 seconds.

Human Jurkat cells probed with Vimentin Polyclonal Antibody, Unconjugated at 0.

This image was kindly submitted by Preethi Vijayaraj from UCLA, 4% PFA fixed cells, permeabilized with 0.

Formalin-fixed and paraffin embedded mouse kidney tissue labeled Anti-Vimentin Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse mesenchymal stem cells labeled Anti-Vimentin Polyclonal Antibody, Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining

E-cadherin, N-cadherin, and vimentin expression in PGCCs with budding and control MCF-7 and MDA-MB-231 cells.

Expression of E-cadherin, N-cadherin, and vimentin in human breast tumor tissues.

Expression of E-cadherin, N-cadherin, and vimentin in single stroma PGCCs of human breast cancer.

13 images

51 references

Catalog No. ABIN672786

Collagen Type I antibody

COL1 Reactivity: Human ELISA Host: Goat Polyclonal unconjugated

Decreases in the levels of activated HSC markers, SOX9, COL1 and phosphoMyl9 (PMYL9) following PAK-1 abrogation by siRNA1 in activated rat HSCs relative to their respective scrambled control levels.

Characterisation of COL1 expression in control and Sox9-null animals following liver fibrosis induction using ABIN135046.

Activated mouse HSCs (‘Control') show decreased protein levels for α-SMA, COL1 and SOX9 by immunoblotting following the loss of integrin beta-1 (‘Itgb1-null').

Elevated collagen levels in skeletal muscles in SGCA- and SGCD-null mice and in the heart of SGCD-null mice investigated using ABIN135046: Immunofluorescent images of skeletal muscles stained with collagen type I (fibrotic marker, green) and laminin (extracellular matrix of muscle fibres, red) Scale bar: 100 μm.

Immunofluorescence images of the heart stained with collagen type I (fibrotic marker, green) using ABIN135046.

(A) Effects on collagen deposition in indirect (inserts) and direct coculture of human dermal fibroblasts and siRNA-treated THP1 cells.

Immunoblot analysis of primary AF and NP cell lysates from two independent donors, D1 (P0) and D3 (P1), respectively, for COL1A1 (ABIN135046) and COL2A1 (ABIN135048).

Immunoblot Analysis using ABIN135046.

8 images

69 references

Catalog No. ABIN135046

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