anti-Human CAPNL1 antibody for Western Blotting

Recommended CAPNL1 Antibody (supplied by: Log in to see )

Antigen
Calpain 1, Large Subunit (CAPNL1) Antibodies
  • CANP
  • CANP1
  • CANPL1
  • muCANP
  • muCL
  • Capa-1
  • Capa1
  • mu-calpin
  • si:ch211-203k12.4
  • si:dkeyp-101g1.3
  • calpain 1
  • calpain 1, (mu/I) large subunit
  • calpain 1, (mu/I) large subunit b
  • CAPN1
  • Capn1
  • capn1
  • capn1b
Epitope
AA 312-326, Middle Region
5
5
4
3
3
3
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
54
15
14
3
2
2
2
2
2
2
1
1
Host
Rabbit
31
23
6
Clonality
Polyclonal
Conjugate
This CAPNL1 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
60
35
16
6
5
5
3
2
1
1
Options
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Catalog No. ABIN3044292
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2802167 ABIN2788625 IHC WB Rabbit Middle Region Log in to see Polyclonal 0
3.2802167 ABIN2788626 WB Rabbit N-Term Log in to see Polyclonal 0
3.2802167 ABIN3043467 IHC (p) WB Rabbit IgG AA 396-555 Log in to see Polyclonal 2
3.2802167 ABIN947641 IF IP WB Rabbit AA 1-714, full length Log in to see Polyclonal 0
3.2802167 ABIN1871429 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2802167 ABIN1858230 ICC IHC WB Rabbit IgG AA 426-676 Log in to see Polyclonal 0
3.2802167 ABIN514000 IF WB Mouse AA 1-714, full length Log in to see Polyclonal 0
3.2802167 ABIN3021623 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2802167 ABIN514001 IF PLA WB Rabbit AA 1-714, full length Log in to see Polyclonal 0
3.2802167 ABIN926628 WB Rabbit Middle Region Log in to see Polyclonal 0
3.2802167 ABIN560161 ELISA WB Mouse AA 610-714, partial Log in to see Polyclonal 0
3.2802167 ABIN926629 WB Rabbit N-Term Log in to see Polyclonal 0
3.2802167 ABIN2561556 ELISA WB Goat IgG AA 468-481 Log in to see Polyclonal 0
3.2802167 ABIN2561555 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
3.2802167 ABIN4907386 IHC (p) WB Mouse IgG1 kappa Log in to see 1376CT809-24-85 0
3.2802167 ABIN2421297 ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.2802167 ABIN2427867 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1590076 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN1590119 ELISA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN5687713 ELISA WB Goat Internal Region Log in to see Polyclonal 0

General

Antigen Calpain 1, Large Subunit (CAPNL1) Antibodies
Epitope AA 312-326, Middle Region
(5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(54), (15), (14), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(31), (23), (6)
Clonality
Polyclonal
Conjugate This CAPNL1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(60), (35), (16), (6), (5), (5), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-CAPNL1 Antibody

Target Details CAPNL1 Application Details Handling References for anti-CAPNL1 antibody (ABIN3044292) Images
Purpose Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Sequence EWNNVDPYER DQLRV
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: calpain 1,(mu/I) large subunit
Protein Name: Calpain-1 catalytic subunit
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.
Isotype IgG

Target Details CAPNL1

Product Details anti-CAPNL1 Antibody Application Details Handling References for anti-CAPNL1 antibody (ABIN3044292) Images back to top
Antigen
Alternative Name CAPN1 (CAPNL1 Antibody Abstract)
Background Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

Synonyms: Ca2 activated neutral protease antibody|Calcium activated neutral proteinase 1 antibody|Calcium activated neutral proteinase antibody|Calcium activated neutral proteinase small subunit antibody|Calcium dependent protease small subunit 1 antibody|Calcium dependent protease small subunit antibody|Calcium-activated neutral proteinase 1 antibody|Calpain 1(mu/I) large subunit antibody|Calpain 1 antibody|Calpain 1 catalytic subunit antibody|Calpain 1 large subunit antibody|Calpain Large Polypeptide L1 antibody|Calpain Large Polypeptide L1 antibody|Calpain mu type antibody|Calpain mu-type antibody|Calpain regulatory subunit antibody|Calpain small subunit 1 antibody|Calpain-1 catalytic subunit antibody|Calpain-1 large subunit antibody|Calpain1 antibody|CAN1_HUMAN antibody|CANP 1 antibody|CANP antibody|CANP small subunit antibody|CANPL 1 antibody|CANPL1 antibody|CAPN 1 antibody|CAPN1 antibody|Cell proliferation inducing protein 30 antibody|Cell proliferation-inducing gene 30 protein antibody|Micromolar Calpain antibody|Micromolar-calpain antibody|Mu Calpain antibody|muCANP antibody|muCL antibody
UniProt P07384
Pathways Apoptosis

Application Details

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Handling References for anti-CAPNL1 antibody (ABIN3044292) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details References for anti-CAPNL1 antibody (ABIN3044292) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

References for anti-CAPNL1 antibody (ABIN3044292)

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details Handling Images back to top
Product cited in:

Chen, Tang, Gao, Xu, Jiang, Cao, Fu, Sun, Liu, Shi: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." in: Medicine, Vol. 93, Issue 23, pp. e138, 2014

Mo, Chen, Li, Zheng, Ke, Deng, Li, Jiang, Wu, Wang, Wang, Yang, Cao: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." in: Microvascular research, Vol. 81, Issue 2, pp. 160-8, 2011

Images

Product Details anti-CAPNL1 Antibody Target Details CAPNL1 Application Details Handling References for anti-CAPNL1 antibody (ABIN3044292) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Calpain 1, Large Subunit (CAPNL1) (AA 312-326), (Middle Region) antibody (ABIN3044292) Anti-Calpain 1 antibody, IHC(P) IHC(P): Rat Lung Tissue
Immunohistochemistry (IHC) image for anti-Calpain 1, Large Subunit (CAPNL1) (AA 312-326), (Middle Region) antibody (ABIN3044292) Anti-Calpain 1 antibody, ICC ICC: HELA Cell
Western Blotting (WB) image for anti-Calpain 1, Large Subunit (CAPNL1) (AA 312-326), (Middle Region) antibody (ABIN3044292) Anti-Calpain 1 antibody, Western blotting Lane 1: Cell Lysate Lane 2: COLO320 Cell L...
Immunohistochemistry (IHC) image for anti-Calpain 1, Large Subunit (CAPNL1) (AA 312-326), (Middle Region) antibody (ABIN3044292) Anti-Calpain 1 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue