anti-Mouse (Murine) Caspase 1 antibody for Immunohistochemistry

Recommended Caspase 1 Antibody (supplied by: Log in to see )

Antigen
Caspase 1 (CASP1) Antibodies
  • ICE
  • IL1BC
  • P45
  • Il1bc
  • Ice
  • CASP1
  • CG5370
  • DCP-1
  • DCP1
  • Dcp1
  • Dmel\\CG5370
  • ccp1
  • dcp-1
  • dcp1
  • l(2)01862
  • l(2)02132
  • caspase 1
  • caspase 1, apoptosis-related cysteine peptidase
  • caspase 4, apoptosis-related cysteine peptidase
  • Death caspase-1
  • caspase-1
  • caspase
  • caspase recruitment domain-containing protein 16
  • caspase 4
  • CASP1
  • Casp1
  • CASP4
  • Dcp-1
  • Caspase1
  • LOC5564196
  • LOC451519
Reactivity
Human, Mouse (Murine), Rat (Rattus)
258
95
74
8
2
2
2
1
1
1
1
1
1
Host
Mouse
250
36
8
2
Clonality
Monoclonal
Conjugate
This Caspase 1 antibody is un-conjugated
23
16
8
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
266
160
118
75
32
26
18
15
7
5
3
2
1
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Catalog No. ABIN252521
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.85804 ABIN1532666 IHC ELISA WB Rabbit IgG AA 342-391 Log in to see Polyclonal 3
8.85804 ABIN1531644 IHC ELISA WB Rabbit IgG AA 342-391, pSer376 Log in to see Polyclonal 3
8.85804 ABIN2705671 IHC WB Rabbit Center Log in to see Polyclonal 0
8.85804 ABIN213734 IHC IHC (p) WB Mouse IgG1, kappa AA 371-390 Log in to see 14F468 0
8.85804 ABIN266745 IHC (fro) IHC (p) IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN4287999 IHC IHC (p) WB Rabbit cleaved, full length Log in to see Polyclonal 1
1 ABIN3183664 ELISA IHC WB Rabbit IgG Ser197 Log in to see Polyclonal 0
1 ABIN5945465 FACS IHC IHC (p) WB Rabbit IgG C-Term Log in to see SU40-07 0
1 ABIN2888870 ELISA IHC IHC (p) WB Rabbit IgG AA 342-391 Log in to see Polyclonal 0
1 ABIN1077907 ICC IHC WB Rabbit IgG AA 119-296 Log in to see Polyclonal 0
1 ABIN3182631 ELISA IHC WB Rabbit IgG pSer376 Log in to see Polyclonal 0
1 ABIN4288024 IHC IHC (p) SimWes WB Mouse IgG1 kappa Log in to see 14F468 0
1 ABIN1732658 IHC (p) IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6260470 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2883562 ELISA IHC IHC (p) WB Rabbit IgG pSer376 Log in to see Polyclonal 0
1 ABIN2891943 IHC IHC (p) WB Rabbit Lys372 Log in to see Polyclonal 0
1 ABIN3021171 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4288008 IHC IHC (p) WB Biotin Mouse IgG1 kappa Log in to see 14F468 0
1 ABIN4288016 IHC IHC (p) WB DyLight 550 Mouse IgG1 kappa Log in to see 14F468 0
1 ABIN4288018 IHC IHC (p) WB DyLight 405 Mouse IgG1 kappa Log in to see 14F468 0

General

Antigen Caspase 1 (CASP1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(258), (95), (74), (8), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(250), (36), (8), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Caspase 1 antibody is un-conjugated
(23), (16), (8), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(266), (160), (118), (75), (32), (26), (18), (15), (7), (5), (3), (2), (1)
Pubmed 25 references available
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Product Details anti-Caspase 1 Antibody

Target Details Caspase 1 Application Details Handling References for anti-Caspase 1 antibody (ABIN252521) Images
Specificity The antibody will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.
Purification Protein G purified
Immunogen A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human caspase-1 was used as immunogen.
Clone 14F468
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Caspase 1

Product Details anti-Caspase 1 Antibody Application Details Handling References for anti-Caspase 1 antibody (ABIN252521) Images back to top
Antigen
Alternative Name Caspase-1 (CASP1 Antibody Abstract)
Background Gene Symbol: CASP1
Molecular Weight Theoretical MW: 45 kDa
Gene ID 834
UniProt P29466
Pathways Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-Caspase 1 Antibody Target Details Caspase 1 Handling References for anti-Caspase 1 antibody (ABIN252521) Images back to top
Application Notes Western Blot 0.5-2 μg/mL, Simple Western 1:50, Immunohistochemistry 1:20-1:1000, Immunohistochemistry-Paraffin 1:10-1:500Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Caspase 1 Antibody Target Details Caspase 1 Application Details References for anti-Caspase 1 antibody (ABIN252521) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Caspase 1 antibody (ABIN252521)

Product Details anti-Caspase 1 Antibody Target Details Caspase 1 Application Details Handling Images back to top
Product cited in:

de Rivero Vaccari, Brand, Adamczak, Lee, Perez-Barcena, Wang, Bullock, Dietrich, Keane: "Exosome-mediated inflammasome signaling after central nervous system injury." in: Journal of neurochemistry, Vol. 136 Suppl 1, pp. 39-48, 2016 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Lin, Li, Zha, Xu, Pan, Zhao, Ouyang, He: "Gossypol induces pyroptosis in mouse macrophages via a non-canonical inflammasome pathway." in: Toxicology and applied pharmacology, Vol. 292, pp. 56-64, 2016 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lévesque, Paré, Mailhot, Bellver-Landete, Kébir, Lécuyer, Alvarez, Prat, de Rivero Vaccari, Keane, Lacroix: "Myeloid cell transmigration across the CNS vasculature triggers IL-1β-driven neuroinflammation during autoimmune encephalomyelitis in mice." in: The Journal of experimental medicine, Vol. 213, Issue 6, pp. 929-49, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Fernandez, Miller, Krammer, Gopal, Greenbaum, Bhardwaj: "Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells." in: Journal of leukocyte biology, Vol. 99, Issue 5, pp. 723-34, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Rimessi, Bezzerri, Patergnani, Marchi, Cabrini, Pinton: "Mitochondrial Ca2+-dependent NLRP3 activation exacerbates the Pseudomonas aeruginosa-driven inflammatory response in cystic fibrosis." in: Nature communications, Vol. 6, pp. 6201, 2015 (Sample species: Human). Further details: Western Blotting

Queen, Agarwal, Dolores, Stehlik, Satchell: "Mechanisms of inflammasome activation by Vibrio cholerae secreted toxins vary with strain biotype." in: Infection and immunity, Vol. 83, Issue 6, pp. 2496-506, 2015 (Sample species: Human). Further details: Western Blotting

Melehani, James, DuMont, Torres, Duncan: "Staphylococcus aureus Leukocidin A/B (LukAB) Kills Human Monocytes via Host NLRP3 and ASC when Extracellular, but Not Intracellular." in: PLoS pathogens, Vol. 11, Issue 6, pp. e1004970, 2015 (Sample species: Human). Further details: Western Blotting

Paciello, Silipo, Lembo-Fazio, Curcurù, Zumsteg, Noël, Ciancarella, Sturiale, Molinaro, Bernardini: "Intracellular Shigella remodels its LPS to dampen the innate immune recognition and evade inflammasome activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 46, pp. E4345-54, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Mawhinney, de Rivero Vaccari, Dale, Keane, Bramlett: "Heightened inflammasome activation is linked to age-related cognitive impairment in Fischer 344 rats." in: BMC neuroscience, Vol. 12, pp. 123, 2012 (Sample species: Rat (Rattus)). Further details: Western Blotting

de Rivero Vaccari, Bastien, Yurcisin, Pineau, Dietrich, De Koninck, Keane, Lacroix: "P2X4 receptors influence inflammasome activation after spinal cord injury." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 9, pp. 3058-66, 2012

de Rivero Vaccari, Sawaya, Brand, Nusbaum, Bauman, Bramlett, Dietrich, Keane: "Caspase-1 level is higher in the scalp in androgenetic alopecia." in: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], Vol. 38, Issue 7 Pt 1, pp. 1033-9, 2012

Tomura, de Rivero Vaccari, Keane, Bramlett, Dietrich: "Effects of therapeutic hypothermia on inflammasome signaling after traumatic brain injury." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 32, Issue 10, pp. 1939-47, 2012

Taxman, Swanson, Broglie, Wen, Holley-Guthrie, Huang, Callaway, Eitas, Duncan, Ting: "Porphyromonas gingivalis mediates inflammasome repression in polymicrobial cultures through a novel mechanism involving reduced endocytosis." in: The Journal of biological chemistry, Vol. 287, Issue 39, pp. 32791-9, 2012

Zhang, Ibrahim, de Rivero Vaccari, Lotocki, Aballa, Dietrich, Keane, Lynne, Brackett: "Involvement of the inflammasome in abnormal semen quality of men with spinal cord injury." in: Fertility and sterility, Vol. 99, Issue 1, pp. 118-24, 2012

Atay, Gercel-Taylor, Taylor: "Human trophoblast-derived exosomal fibronectin induces pro-inflammatory IL-1β production by macrophages." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 66, Issue 4, pp. 259-69, 2011

Taxman, Holley-Guthrie, Huang, Moore, Bergstralh, Allen, Lei, Gris, Ting: "The NLR adaptor ASC/PYCARD regulates DUSP10, mitogen-activated protein kinase (MAPK), and chemokine induction independent of the inflammasome." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19605-16, 2011

Nadeau, Filali, Zhang, Kerr, Rivest, Soulet, Iwakura, de Rivero Vaccari, Keane, Lacroix: "Functional recovery after peripheral nerve injury is dependent on the pro-inflammatory cytokines IL-1β and TNF: implications for neuropathic pain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 35, pp. 12533-42, 2011 (Sample species: Mouse (Murine)).

Rawat, Cohen, Ampong, Francia, Henriques-Pons, Hoffman, Nagaraju: "Inflammasome up-regulation and activation in dysferlin-deficient skeletal muscle." in: The American journal of pathology, Vol. 176, Issue 6, pp. 2891-900, 2010

Banerjee, Datta, Majumder, Mukhopadhyay, Bhattacharyya: "Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1." in: Nucleic acids research, Vol. 38, Issue 3, pp. 878-92, 2010

Hurst, Prinz, Lorenz, Bauer, Chapman, Lackner, von Landenberg: "TLR7 and TLR8 ligands and antiphospholipid antibodies show synergistic effects on the induction of IL-1beta and caspase-1 in monocytes and dendritic cells." in: Immunobiology, Vol. 214, Issue 8, pp. 683-91, 2009

Images

Product Details anti-Caspase 1 Antibody Target Details Caspase 1 Application Details Handling References for anti-Caspase 1 antibody (ABIN252521) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Western blot analysis of Caspase-1 in A) HeLa cell lysate at 0.5 ug/ml and B) NIH 3T3...
Immunohistochemistry (IHC) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Formalin-fixed, paraffin-embedded sections of human colon cancer stained for caspase-...
Immunohistochemistry (IHC) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) anti-Caspase 1 (CASP1) antibody (Image 3)
Western Blotting (WB) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Western Blot: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis using Azide and BS...
Simple Western (SimWes) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Simple Western: Caspase-1 Antibody (14F468) [ABIN2525215] - Simple Western lane view ...
Simple Western (SimWes) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Simple Western: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis using Azide and ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Detection ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - IHC-P dete...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Detection ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - IHC-P dete...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Analysis u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) [ABIN2525215] - Formalin-f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 1 (CASP1) antibody (ABIN252521) Immunohistochemistry-Paraffin: Caspase-1 Antibody (14F468) - IHC analysis of a forma...