anti-Human TNFSF10 antibody for Flow Cytometry

Recommended TNFSF10 Antibody (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) Antibodies
  • APO2L
  • Apo-2L
  • CD253
  • TL2
  • TRAIL
  • A330042I21Rik
  • AI448571
  • APO-2L
  • Ly81
  • Trail
  • tl2
  • apo2l
  • cd253
  • trail
  • apo-2l
  • trail1
  • xtrail1
  • tnfsf10l2
  • zgc:92320
  • TNLG6A
  • TNF superfamily member 10
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor superfamily member 10
  • TNF superfamily member 10 S homeolog
  • TNFSF10
  • Tnfsf10
  • tnfsf10.S
  • tnfsf10
Reactivity
Human
333
81
37
4
3
2
1
1
1
Host
Mouse
210
156
24
2
Clonality
Monoclonal
Conjugate
This TNFSF10 antibody is un-conjugated
42
21
16
10
9
5
4
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Biochemical Assay (BCA), Blocking Reagent (BR), Flow Cytometry (FACS)
261
132
114
62
53
28
19
15
15
13
11
10
9
5
3
3
2
2
1
1
1
1
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Catalog No. ABIN2689129
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.762436 ABIN1106154 FACS PE Mouse IgG1 Log in to see B-S23 1
13.762436 ABIN4897836 FACS PE Mouse IgG1 AA 95-281 Log in to see 75402 3
13.762436 ABIN1106153 FACS Biotin Mouse IgG1 Log in to see B-S23 0
13.762436 ABIN2661009 FACS Biotin Mouse IgG1 kappa Log in to see RIK-2 8
13.762436 ABIN2663220 FACS PE Mouse IgG1 kappa Log in to see RIK-2 9
13.762436 ABIN4897834 FACS APC Mouse IgG1 AA 95-281 Log in to see 75402 0
13.762436 ABIN4897835 FACS Alexa Fluor 488 Mouse IgG1 AA 95-281 Log in to see 75402 0
13.762436 ABIN262794 FACS Mouse IgG1 kappa Log in to see RIK-2 0
1 ABIN2689127 FACS Mouse IgG1 Log in to see RIK 6
1 ABIN2689130 FACS Biotin Mouse IgG1 Log in to see RIK 5
1 ABIN2751999 Func FACS Mouse IgG1 AA 95-281 Log in to see 2E5 2
1 ABIN2749022 Func FACS Mouse IgG1 AA 95-281 Log in to see 2E5 1
1 ABIN1383744 FACS PE Mouse IgG1 Log in to see B-S23 3
1 ABIN1690743 Func FACS PE Mouse IgG1 AA 95-281 Log in to see 2E5 2
1 ABIN4361856 ELISA FACS IHC IHC (fro) IP WB Mouse IgG2b Log in to see III6F 2
1 ABIN473970 FACS PE Mouse IgG1, kappa Log in to see RIK-2 0
1 ABIN475703 FACS Biotin Mouse IgG1, kappa Log in to see RIK-2 0
1 ABIN475921 FACS Mouse IgG1, kappa Log in to see RIK-2 0
1 ABIN1383746 FACS Biotin Mouse IgG1 Log in to see B-S23 0
1 ABIN2665611 FACS IHC Mouse IgG1 kappa Log in to see RIK-2 8

General

Antigen Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) Antibodies
Reactivity Human
(333), (81), (37), (4), (3), (2), (1), (1), (1)
Host Mouse
(210), (156), (24), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This TNFSF10 antibody is un-conjugated
(42), (21), (16), (10), (9), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Biochemical Assay (BCA), Blocking Reagent (BR), Flow Cytometry (FACS)
(261), (132), (114), (62), (53), (28), (19), (15), (15), (13), (11), (10), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-TNFSF10 Antibody

Target Details TNFSF10 Application Details Handling References for anti-TNFSF10 antibody (ABIN2689129) Images
Brand BD Pharmingen™
Characteristics The RIK-2 monoclonal antibody specifically binds to CD253 which is also known as TRAIL (TNF-Related Apoptosis-Inducing Ligand) and Apo2 ligand (APO-2L). CD253 is a member of the Tumor Necrosis Factor Superfamily and is encoded by the TNFSF10 gene. CD253 is a type II membrane protein that may be expressed as a full-length, cell surface associated protein as well as in a soluble form. Both surface and soluble forms of TRAIL rapidly induce apoptosis by a wide range of cell lines but not by normal cells. TRAIL is expressed by activated T cells, NK cells, dendritic cells, monocytes and by a variety of non-lymphoid cells. TRAIL can bind to and exert apoptosis through DR4 (TRAIL-R1) and DR5 (TRAIL-R2) receptors. It can also bind to decoy receptors, including DcR1/TRID/TRAIL-R3 and DcR2/TRUNDD/TRAIL-R4, and possibly OPG/TNFRSF11B, which may serve to regulate TRAIL activity. TRAIL has been shown to be involved in T cell-mediated cytotoxicity, however its mechanism of action remains to be fully elucidated. The RIK-2 clone was selected based on its ability to block TRAIL-mediated cytotoxic activity. Flow Cytometric Analysis of TRAIL-induced Killing and RIK-2 Blocking using FITC Annexin V staining. Jurkat T cells were left untreated (far left/first panel) or treated for 16 hours under the following conditions: Cells were incubated with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody (second panel), or with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody preincubated with RIK-2 antibody (third panel), or with 20 ng/mL of recombinant human TRAIL and 10 μg/mL anti-histidine antibody preincubated with mIgG1 antibody (negative control) (far right/fourth panel). Following treatments, cells were evaluated for Annexin-V staining. Cells induced to undergo apoptosis by treatment with recombinant human TRAIL gave a population of cells that was Annexin V-FITC positive (second panel, M2). Annexin V-FITC staining was blocked when cells were incubated with a mixture of recombinant human TRAIL and RIK-2 antibody (third panel, M2). Cells treated with the mixture of recombinant TRAIL and mIgG1 antibody could not block the killing (far right/fourth panel). A small number of Annexin-V positive cells in the untreated population represents a basal level of apoptosis (far left/first panel). The results indicate that clone RIK-2 can block cell mediated killing induced by recombinant human TRAIL as measured by Annexin V-FITC staining of Jurkat cells.

BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD253 - NA/LE - Clone RIK-2 - Isotype Mouse IgG1 - Reactivity Hu - 0.25 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility Sterile filtered,0.2 μm filtered
Endotoxin Level Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Human TRAIL Transfected Cell Line
Clone RIK
Isotype IgG1

Target Details TNFSF10

Product Details anti-TNFSF10 Antibody Application Details Handling References for anti-TNFSF10 antibody (ABIN2689129) Images back to top
Antigen
Alternative Name CD253 (TNFSF10 Antibody Abstract)
Background Synonyms: TNFSF10, TRAIL, APO-2L, TL2
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-TNFSF10 Antibody Target Details TNFSF10 Handling References for anti-TNFSF10 antibody (ABIN2689129) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-TNFSF10 Antibody Target Details TNFSF10 Application Details References for anti-TNFSF10 antibody (ABIN2689129) Images back to top
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Without preservative
Handling Advice This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-TNFSF10 antibody (ABIN2689129)

Product Details anti-TNFSF10 Antibody Target Details TNFSF10 Application Details Handling Images back to top
Product cited in:

Kayagaki, Yamaguchi, Nakayama, Kawasaki, Akiba, Okumura, Yagita: "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2639-47, 1999

Marsters, Pitti, Donahue, Ruppert, Bauer, Ashkenazi: "Activation of apoptosis by Apo-2 ligand is independent of FADD but blocked by CrmA." in: Current biology : CB, Vol. 6, Issue 6, pp. 750-2, 1997

Sheridan, Marsters, Pitti, Gurney, Skubatch, Baldwin, Ramakrishnan, Gray, Baker, Wood, Goddard, Godowski, Ashkenazi: "Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors." in: Science (New York, N.Y.), Vol. 277, Issue 5327, pp. 818-21, 1997

Pitti, Marsters, Ruppert, Donahue, Moore, Ashkenazi: "Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family." in: The Journal of biological chemistry, Vol. 271, Issue 22, pp. 12687-90, 1996

Wiley, Schooley, Smolak, Din, Huang, Nicholl, Sutherland, Smith, Rauch, Smith: "Identification and characterization of a new member of the TNF family that induces apoptosis." in: Immunity, Vol. 3, Issue 6, pp. 673-82, 1996

Images

Product Details anti-TNFSF10 Antibody Target Details TNFSF10 Application Details Handling References for anti-TNFSF10 antibody (ABIN2689129) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) antibody (ABIN2689129) anti-Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10) antibody