anti-Human Insulin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Insulin Antibody (supplied by: Log in to see )

Antigen
Insulin (INS) Antibodies
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)
1064
575
463
453
249
236
47
9
7
6
5
5
4
2
2
1
1
1
1
1
1
1
Host
Mouse
895
195
50
16
3
1
Clonality
Monoclonal
Conjugate
This Insulin antibody is conjugated to CF®405S
58
48
38
28
27
22
22
20
19
17
17
14
14
14
14
14
14
14
10
10
10
10
10
10
10
10
10
10
10
10
9
9
9
9
8
8
8
5
5
5
5
5
5
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
496
405
363
329
272
233
190
189
118
67
66
38
31
26
23
8
7
6
5
4
4
3
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6157329
$ 157.54
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN6157332 FACS IF IHC (f) CF405M Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157331 FACS IF IHC (f) CF405M Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157338 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157337 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157340 FACS IF IHC (f) CF®555 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157342 FACS IF IHC (f) CF®660R Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157344 FACS IF IHC (f) CF®568 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157345 FACS IF IHC (f) CF®770 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157370 FACS IF IHC (f) CF405M Mouse IgG Log in to see E2-E3 2D11-H5 0
1 ABIN6157409 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157410 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157407 FACS IF IHC (f) CF405M Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157414 FACS IF IHC (f) CF®647 Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157420 FACS IF IHC (f) CF®568 Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157428 FACS IF IHC (f) CF®488A Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157430 FACS IF IHC (f) CF®594 Mouse IgG1, kappa Log in to see E2-E3 0
1 ABIN6157444 FACS IF IHC (f) CF®405S Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157448 FACS IF IHC (f) CF®640R Mouse IgG1, kappa Log in to see IRDN-794 0
1 ABIN6157335 FACS IF IHC (f) CF®543 Mouse IgG1, kappa Log in to see 2D11-H5 0
1 ABIN6157346 FACS IF IHC (f) CF®770 Mouse IgG1, kappa Log in to see 2D11-H5 0

General

Antigen Insulin (INS) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)
(1064), (575), (463), (453), (249), (236), (47), (9), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(895), (195), (50), (16), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Insulin antibody is conjugated to CF®405S
(58), (48), (38), (28), (27), (22), (22), (20), (19), (17), (17), (14), (14), (14), (14), (14), (14), (14), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (9), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(496), (405), (363), (329), (272), (233), (190), (189), (118), (67), (66), (38), (31), (26), (23), (8), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-Insulin Antibody

Target Details Insulin Application Details Handling Images
Purpose Mouse Monoclonal anti-Insulin (2D11-H5), CF405S Conjugate
Characteristics This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Purified pig insulin, conjugated to KLH
Clone 2D11-H5
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details Insulin

Product Details anti-Insulin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Insulin (INS Antibody Abstract)
Background IDDM2, ILPR, IRDN, MODY10, Proinsulin
Molecular Weight 6 kDa
Gene ID 3630, 272259
UniProt P01308
Pathways NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-Insulin Antibody Target Details Insulin Handling Images back to top
Application Notes Immunohistology formalin-fixed 0.1-0.2 μg/mL
  • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Immunofluorescence 1-2 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

MIA PaCa-2 cells or Pancreas

Restrictions For Research Use only

Handling

Product Details anti-Insulin Antibody Target Details Insulin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from light