anti-Human CDKN1B antibody for Immunocytochemistry

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
Center
81
56
47
37
29
29
25
20
19
19
19
19
17
16
14
13
10
8
7
7
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
778
423
359
182
40
25
19
8
8
7
3
3
2
1
1
1
Host
Rabbit
470
306
39
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
40
35
35
34
33
29
9
7
7
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
629
338
241
230
199
196
102
92
84
52
37
17
14
5
5
3
1
1
1
Options
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Catalog No. ABIN2854753
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN6271268 ELISA ICC IF IHC WB Rabbit IgG pThr157 Log in to see Polyclonal 0
19 ABIN6271270 ELISA ICC IF IHC WB Rabbit IgG pSer178 Log in to see Polyclonal 0
13.25679 ABIN6138338 FACS ICC IHC IP WB Rabbit Log in to see Polyclonal 0
13 ABIN6256493 ELISA ICC IF IHC WB Rabbit IgG pThr187 Log in to see Polyclonal 0
11.5 ABIN6263944 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 2
10 ABIN6254921 ELISA ICC IF WB Rabbit IgG pSer10 Log in to see Polyclonal 0
7 ABIN6256498 ELISA ICC IF WB Rabbit IgG pThr198 Log in to see Polyclonal 0
6.9755297 ABIN442186 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 0
6.9755297 ABIN5949261 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 150-250 Log in to see SU37-04 0
5.5 ABIN3043662 ICC WB Mouse IgG1 Log in to see DCS-72 1
4 ABIN2737293 ICC FACS IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1501969 ICC WB Mouse IgG1 Log in to see IMD-27 0
1 ABIN596894 ICC WB Mouse IgG1 Log in to see 0
1 ABIN2594381 ICC IF IHC IHC (p) WB Rabbit pThr187 Log in to see Polyclonal 0
1 ABIN3030636 ICC WB Mouse IgG1 Log in to see DCS-72-aka IMD-27 0
-5.0244703 ABIN5078560 ICC IF WB Rabbit IgG Log in to see Polyclonal 0
-11.02447 ABIN4342495 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SX53G8 0
-11.02447 ABIN4342498 FACS ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see SPM348 0
-11.02447 ABIN5078558 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 1-198 Log in to see 7B9 0
-11.02447 ABIN4342508 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see SX53G8 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope Center
(81), (56), (47), (37), (29), (29), (25), (20), (19), (19), (19), (19), (17), (16), (14), (13), (10), (8), (7), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(778), (423), (359), (182), (40), (25), (19), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Rabbit
(470), (306), (39), (2), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1B antibody is un-conjugated
(40), (35), (35), (34), (33), (29), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(629), (338), (241), (230), (199), (196), (102), (92), (84), (52), (37), (17), (14), (5), (5), (3), (1), (1), (1)
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))
p27 Kip1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name p27 Kip1 (CDKN1B Antibody Abstract)
Background This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 22 kDa
Gene ID 1027
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: MCF-7 , NIH-3T3 , CCRF-SB , MFB (mouse fibroblast) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
Format Liquid
Concentration 0.96 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Whole c...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image Sample (50 ug of whole cell lysate) A: CCRF-SB 12% SDS PAGE antibody diluted...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image Sample (50 ug of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE a...
Immunoprecipitation (IP) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) IP Image Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using...
Immunofluorescence (IF) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) ICC/IF Image p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by i...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Various...