anti-Rat (Rattus) CD55 antibody for Enzyme Immunoassay

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • CR
  • CROM
  • DAF
  • TC
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CD55 molecule (Cromer blood group)
  • CD55 molecule, decay accelerating factor for complement
  • CD55
  • Cd55
Reactivity
Rat (Rattus)
463
56
24
10
9
4
3
2
2
2
Host
Mouse
370
117
15
10
8
3
3
Clonality
Monoclonal
Conjugate
This CD55 antibody is un-conjugated
58
50
32
19
10
9
9
8
8
7
5
5
5
5
5
5
5
5
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
375
205
124
106
73
73
43
43
16
13
6
5
5
5
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN5541172
$ 754.40
Plus shipping costs $45.00

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Rat (Rattus)
(463), (56), (24), (10), (9), (4), (3), (2), (2), (2)
Host Mouse
(370), (117), (15), (10), (8), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is un-conjugated
(58), (50), (32), (19), (10), (9), (9), (8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(375), (205), (124), (106), (73), (73), (43), (43), (16), (13), (6), (5), (5), (5), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling Images
Specificity The monoclonal antibody RDII-24 recognizes complement decay accelerating factor (DAF), also designated as CD55.
Purification Purified
Immunogen NIH-3T3 cells expressing GPI-anchored Rat DAF
Clone RDIII-24
Isotype IgG1
Plasmids, Primers & others

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling Images back to top
Antigen
Alternative Name cd55,daf (CD55 Antibody Abstract)
Background Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement-mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.
UniProt Q9QYJ9
Pathways Complement System, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling Images back to top
Application Notes Flow Cytometry (Ref.1,2). Western blot: Non-reduced with a band size of 60-70 kDa. As positive control CHO-Rat DAF hyper-expressing cells were used (Ref.1,2). Functional Studies: RDIII-7 antibody completely blocked the protective effect of expressed Rat DAF (Ref.2). Immuno Assays (Ref.3). Immunohistochemistry on Frozen Sections (Ref.2). The typical starting working dilution is 1/50.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details Images back to top
Format Liquid
Buffer Buffer System: PBS Stabilizers: 0.1 % BSA
Storage 4 °C
Storage Comment Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Expiry Date 12 months