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General |
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Antigen | Complement Decay-Accelerating Factor (CD55) Antibodies |
Reactivity | Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This CD55 antibody is un-conjugated Alternatives |
Application |
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Alternatives
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Supplier | Log in to see |
Product Details anti-CD55 AntibodyTarget Details CD55 Application Details Handling Images |
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Specificity | The monoclonal antibody RDII-24 recognizes complement decay accelerating factor (DAF), also designated as CD55. |
Purification | Purified |
Immunogen | NIH-3T3 cells expressing GPI-anchored Rat DAF |
Clone | RDIII-24 |
Isotype | IgG1 |
Target Details CD55Product Details anti-CD55 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | cd55,daf (CD55 Antibody Abstract) |
Background | Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement-mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. |
UniProt | Q9QYJ9 |
Pathways | Complement System, Regulation of Leukocyte Mediated Immunity |
Application DetailsProduct Details anti-CD55 Antibody Target Details CD55 Handling Images back to top |
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Application Notes | Flow Cytometry (Ref.1,2). Western blot: Non-reduced with a band size of 60-70 kDa. As positive control CHO-Rat DAF hyper-expressing cells were used (Ref.1,2). Functional Studies: RDIII-7 antibody completely blocked the protective effect of expressed Rat DAF (Ref.2). Immuno Assays (Ref.3). Immunohistochemistry on Frozen Sections (Ref.2). The typical starting working dilution is 1/50. |
Restrictions | For Research Use only |
HandlingProduct Details anti-CD55 Antibody Target Details CD55 Application Details Images back to top |
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Format | Liquid |
Buffer | Buffer System: PBS Stabilizers: 0.1 % BSA |
Storage | 4 °C |
Storage Comment | Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch. |
Expiry Date | 12 months |