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|Conjugate||This CD59 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
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Product Details anti-CD59 AntibodyTarget Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN118747) Images
|Specificity||This antibody reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. MEM-43 has been shown to stimulate NK activity.|
|Purification||Affinity chromatography on Protein A|
Target Details CD59Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 antibody (ABIN118747) Images back to top
|Alternative Name||CD59 (CD59 Antibody Abstract)|
|Background||The antigen is expressed on many haemapoietic and non-haemapoietic cells.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin|
|Research Area||Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 antibody (ABIN118747) Images back to top
Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot: The epitope recognised by MEM-43 is lost after reduction, therefore, non- reducing conditions are required for western blotting techniques. Immunohistochemistry on frozen and paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 antibody (ABIN118747) Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.|
References for anti-CD59 antibody (ABIN118747)Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Tandon, Yan, Morgan, Weetman: "Expression and function of multiple regulators of complement activation in autoimmune thyroid disease." in: Immunology, Vol. 81, Issue 4, pp. 643-7, 1994
Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991
Stefanová, Hilgert, Kristofová, Brown, Low, Horejsí: "Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol." in: Molecular immunology, Vol. 26, Issue 2, pp. 153-61, 1989
Horejsí, Angelisová, Bazil, Kristofová, Stoyanov, Stefanová, Hausner, Vosecký, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988
ImagesProduct Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN118747) back to top