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|Antigen||Complement Component Receptor 1 (CD35) (CR1) Antibodies|
|Reactivity||Baboon, Human, Monkey Alternatives|
|Conjugate||This CR1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
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Product Details anti-CR1 AntibodyTarget Details CR1 Application Details Handling References for anti-CR1 antibody (ABIN118609) Images
|Purification||Affinity Chromatography on Protein A|
|Immunogen||Human acute monocytic leukaemia cells. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.|
Target Details CR1Product Details anti-CR1 Antibody Application Details Handling References for anti-CR1 antibody (ABIN118609) Images back to top
|Alternative Name||CD35 / C3BR (CR1 Antibody Abstract)|
|Background||CD35 is a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1|
|Pathways||Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity|
Application DetailsProduct Details anti-CR1 Antibody Target Details CR1 Handling References for anti-CR1 antibody (ABIN118609) Images back to top
Flow Cytometry: 1/50 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1 μg/mL - 10 μg/mL. Immunohistochemistry on paraffin sections: 1 μg/mL - 10 μg/mL, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CR1 Antibody Target Details CR1 Application Details References for anti-CR1 antibody (ABIN118609) Images back to top
|Buffer||PBS, pH 7.4, 0.09 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CR1 antibody (ABIN118609)Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling Images back to top
Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000
Sopper, Stahl-Hennig, Demuth, Johnston, Dörries, ter Meulen: "Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, 1998
Birmingham, Logar, Shen, Chen: "The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2586-92, 1996
Hogg, Ross, Jones, Slusarenko, Walport, Lachmann: "Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1)." in: European journal of immunology, Vol. 14, Issue 3, pp. 236-43, 1984
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