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|Antigen||Leucyl-tRNA Synthetase (LARS) Antibodies|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
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Product Details anti-LARS AntibodyTarget Details LARS Application Details Handling References for anti-LARS antibody (ABIN359572) Images
|Specificity||This antibody reacts to LARS.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LARS.|
|Plasmids, Primers & others|
Target Details LARSProduct Details anti-LARS Antibody Application Details Handling References for anti-LARS antibody (ABIN359572) Images back to top
|Alternative Name||LARS (LARS Antibody Abstract)|
|Background||LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.Synonyms: EC=22.214.171.124, KIAA1352, LARS, LeuRS, Leucine-tRNA ligase, Leucyl-tRNA synthetase, cytoplasmic|
|Gene ID||51520, 9606|
|Pathways||EGFR Signaling Pathway|
Application DetailsProduct Details anti-LARS Antibody Target Details LARS Handling References for anti-LARS antibody (ABIN359572) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemsitry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LARS Antibody Target Details LARS Application Details References for anti-LARS antibody (ABIN359572) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-LARS antibody (ABIN359572)Product Details anti-LARS Antibody Target Details LARS Application Details Handling Images back to top
Lue, Kelley: "A single residue in leucyl-tRNA synthetase affecting amino acid specificity and tRNA aminoacylation." in: Biochemistry, Vol. 46, Issue 15, pp. 4466-72, 2007
Ling, Yao, Zheng, Wei, Wang, Wu, Wang: "The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex." in: The Journal of biological chemistry, Vol. 280, Issue 41, pp. 34755-63, 2005
Giles, Shimizu, Ruddle: "Assignment of a human genetic locus to chromosome 5 which corrects the heat sensitive lesion associated with reduced leucyl-tRNA synthetase activity in ts025Cl Chinese hamster cells." in: Somatic cell genetics, Vol. 6, Issue 5, pp. 667-687, 1981
ImagesProduct Details anti-LARS Antibody Target Details LARS Application Details Handling References for anti-LARS antibody (ABIN359572) back to top