anti-Human LARS antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended LARS Antibody (supplied by: Log in to see )

Antigen
Leucyl-tRNA Synthetase (LARS) Antibodies
  • HSPC192
  • LARS1
  • LEURS
  • LEUS
  • LRS
  • PIG44
  • RNTLS
  • hr025Cl
  • CG7479
  • Dmel\\CG7479
  • LeuRS
  • F21M12.1
  • F21M12_1
  • BA4991
  • DDBDRAFT_0183834
  • DDBDRAFT_0231251
  • DDB_0183834
  • DDB_0231251
  • 2310045K21Rik
  • 3110009L02Rik
  • AW536573
  • mKIAA1352
  • leucyl-tRNA synthetase
  • Leucyl-tRNA synthetase, mitochondrial
  • ATP binding/leucine-tRNA ligases/aminoacyl-tRNA ligase
  • leucine--tRNA ligase
  • leucyl-tRNA synthetase LeuS
  • leucyl-tRNA ligase
  • leucine-tRNA ligase
  • LARS
  • LeuRS-m
  • AT1G09620
  • leuS
  • APH_RS02290
  • mleuS
  • Lars
Epitope
C-Term
6
4
3
3
2
2
1
1
Reactivity
Human
24
15
6
2
1
1
1
1
1
1
Host
Rabbit
27
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
26
13
7
5
5
5
3
3
3
1
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Catalog No. ABIN359572
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN391611 IHC (p) WB Rabbit Ig Fraction AA 1118-1145, C-Term Log in to see Polyclonal 3
10 ABIN5531515 IHC (p) WB Rabbit Ig Fraction AA 1118-1145, C-Term Log in to see Polyclonal 0
1 ABIN1805074 IHC IHC (p) WB Rabbit AA 1118-1145 Log in to see Polyclonal 0

General

Antigen Leucyl-tRNA Synthetase (LARS) Antibodies
Epitope C-Term
(6), (4), (3), (3), (2), (2), (1), (1)
Reactivity Human
(24), (15), (6), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(27)
Clonality
Polyclonal
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(26), (13), (7), (5), (5), (5), (3), (3), (3), (1)
Pubmed 3 references available
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Product Details anti-LARS Antibody

Target Details LARS Application Details Handling References for anti-LARS antibody (ABIN359572) Images
Specificity This antibody reacts to LARS.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human LARS.
Isotype Ig Fraction
Plasmids, Primers & others

Target Details LARS

Product Details anti-LARS Antibody Application Details Handling References for anti-LARS antibody (ABIN359572) Images back to top
Antigen
Alternative Name LARS (LARS Antibody Abstract)
Background LARS, a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. This enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain.Synonyms: EC=6.1.1.4, KIAA1352, LARS, LeuRS, Leucine-tRNA ligase, Leucyl-tRNA synthetase, cytoplasmic
Gene ID 51520, 9606
UniProt Q9P2J5
Pathways EGFR Signaling Pathway

Application Details

Product Details anti-LARS Antibody Target Details LARS Handling References for anti-LARS antibody (ABIN359572) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemsitry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LARS Antibody Target Details LARS Application Details References for anti-LARS antibody (ABIN359572) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-LARS antibody (ABIN359572)

Product Details anti-LARS Antibody Target Details LARS Application Details Handling Images back to top
Background publications

Lue, Kelley: "A single residue in leucyl-tRNA synthetase affecting amino acid specificity and tRNA aminoacylation." in: Biochemistry, Vol. 46, Issue 15, pp. 4466-72, 2007

Ling, Yao, Zheng, Wei, Wang, Wu, Wang: "The C-terminal appended domain of human cytosolic leucyl-tRNA synthetase is indispensable in its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex." in: The Journal of biological chemistry, Vol. 280, Issue 41, pp. 34755-63, 2005

Giles, Shimizu, Ruddle: "Assignment of a human genetic locus to chromosome 5 which corrects the heat sensitive lesion associated with reduced leucyl-tRNA synthetase activity in ts025Cl Chinese hamster cells." in: Somatic cell genetics, Vol. 6, Issue 5, pp. 667-687, 1981

Images

Product Details anti-LARS Antibody Target Details LARS Application Details Handling References for anti-LARS antibody (ABIN359572) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Leucyl-tRNA Synthetase (LARS) (C-Term) antibody (ABIN359572) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with LAR...
Western Blotting (WB) image for anti-Leucyl-tRNA Synthetase (LARS) (C-Term) antibody (ABIN359572) Western blot analysis of anti-LARS Antibody (C-term) in 293 cell line lysates (35ug/l...