anti-Mouse (Murine) DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 antibody for Western Blotting

Recommended DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 Antibody (supplied by: Log in to see )

Antigen
DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) Antibodies
  • CHL1
  • CHLR1
  • KRG2
  • WABS
  • 4732462I11Rik
  • essa15a
  • putative ATP-dependent RNA helicase DDX11-like protein 8
  • DEAD/H-box helicase 11
  • DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
  • LOC100448472
  • DDX11
  • Ddx11
Epitope
AA 663-691, Middle Region
11
11
10
8
8
7
7
7
4
2
1
1
1
Reactivity
Human, Mouse (Murine)
74
13
1
Host
Rabbit
65
9
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
58
36
36
13
8
8
6
1
1
Supplier
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Catalog No. ABIN951862
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7.819988 ABIN2995654 FACS WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN5534127 FACS WB Rabbit Ig Fraction AA 656-683 Log in to see Polyclonal 0
4.819988 ABIN654411 FACS WB Rabbit Ig Fraction AA 656-683, Center Log in to see Polyclonal 4
1 ABIN1975967 FACS ELISA WB PE Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1973863 FACS ELISA WB HRP Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1969052 FACS ELISA WB Biotin Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1964953 FACS ELISA WB APC Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1962576 FACS ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1971367 FACS ELISA WB FITC Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN2619979 ELISA FACS WB Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1813509 FACS WB Rabbit AA 656-683 Log in to see Polyclonal 0
1 ABIN6098432 ELISA IHC WB Rabbit IgG AA 1-220 Log in to see Polyclonal 0

General

Antigen DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) Antibodies
Epitope AA 663-691, Middle Region
(11), (11), (10), (8), (8), (7), (7), (7), (4), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(74), (13), (1)
Host Rabbit
(65), (9)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)
(58), (36), (36), (13), (8), (8), (6), (1), (1)
Pubmed 4 references available
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Product details

Target Details Application Details Handling References Images
Specificity This antibody recognizes Human and Mouse DEAD box protein 11 (Center).
Purification Affinity Chromatography on Protein A
Immunogen KLH conjugated synthetic peptide between 663-691 amino acids from the Central region of Human DDX11.
Isotype Ig Fraction

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name DDX11 (DDX11 Antibody Abstract)
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX11 encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. DDX11 is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability.Synonyms: CHL1, CHLR1, DEAD box protein 11, DEAD/H box protein 11, KRG-2, KRG2, Keratinocyte growth factor-regulated gene 2 protein, Probable ATP-dependent RNA helicase DDX11
Molecular Weight 108313 Da
Gene ID 1663
NCBI Accession NP_004390
Pathways ER-Nucleus Signaling

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References

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Background publications

Leman, Noguchi, Lee, Noguchi: "Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion." in: Journal of cell science, Vol. 123, Issue Pt 5, pp. 660-70, 2010

Parish, Bean, Park, Androphy: "ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance." in: Molecular cell, Vol. 24, Issue 6, pp. 867-76, 2006

Parish, Rosa, Wang, Lahti, Doxsey, Androphy: "The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells." in: Journal of cell science, Vol. 119, Issue Pt 23, pp. 4857-65, 2006

Product cited in:

Farina, Shin, Kim, Bermudez, Kelman, Seo, Hurwitz: "Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1." in: The Journal of biological chemistry, Vol. 283, Issue 30, pp. 20925-36, 2008

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Flow Cytometry (FACS) image for anti-DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) (AA 663-691), (Middle Region) antibody (ABIN951862) Flow cytometric analysis of Neuro-2a cells using DDX11 Antibody (Center) Cat.-No AP51...
Western Blotting (WB) image for anti-DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) (AA 663-691), (Middle Region) antibody (ABIN951862) Western blot analysis of DDX11 Antibody (Center) in mouse Neuro-2a cell line lysates ...