anti-Mouse (Murine) DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 antibody for Western Blotting

Recommended DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 Antibody (supplied by: Log in to see )

Antigen
DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) Antibodies
  • CHL1
  • CHLR1
  • KRG2
  • WABS
  • 4732462I11Rik
  • essa15a
  • putative ATP-dependent RNA helicase DDX11-like protein 8
  • DEAD/H-box helicase 11
  • DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
  • LOC100448472
  • DDX11
  • Ddx11
Epitope
AA 656-683, Center
12
11
10
8
8
8
7
7
3
1
1
1
1
Reactivity
Human, Mouse (Murine)
69
12
1
Host
Rabbit
60
9
Clonality
Polyclonal
Conjugate
Un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
52
34
33
13
8
6
6
2
1
Options
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Catalog No. ABIN654411
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0155742 ABIN951862 EIA FACS WB Rabbit Ig Fraction AA 663-691, Middle Region Log in to see Polyclonal 4
1 ABIN5534127 FACS WB Rabbit Ig Fraction AA 656-683 Log in to see Polyclonal 0
1 ABIN1962576 FACS ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1964953 FACS ELISA WB APC Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1973863 FACS ELISA WB HRP Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1969052 FACS ELISA WB Biotin Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1971367 FACS ELISA WB FITC Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1975967 FACS ELISA WB PE Rabbit IgG AA 656-683 Log in to see Polyclonal 0
1 ABIN1813509 FACS WB Rabbit AA 656-683 Log in to see Polyclonal 0
1 ABIN2619979 ELISA FACS WB Rabbit IgG AA 656-683 Log in to see Polyclonal 0

General

Antigen DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) Antibodies
Epitope AA 656-683, Center
(12), (11), (10), (8), (8), (8), (7), (7), (3), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(69), (12), (1)
Host Rabbit
(60), (9)
Clonality (Clone)
Polyclonal   ( )
Conjugate Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(52), (34), (33), (13), (8), (6), (6), (2), (1)
Pubmed 4 references available
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Product details

Target Details Application Details Handling References Images
Specificity This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-691 amino acids from the Central region of human DDX11.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-683 AA from the Central region of human DDX11.
Clone RB27628
Isotype Ig Fraction

Target Details

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Antigen
Alternative Name DDX11 (DDX11 Antibody Abstract)
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX11 encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. DDX11 is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability.
Synonyms: DDX11,Probable ATP-dependent RNA helicase DDX11,CHL1, CHLR1, KRG2
Molecular Weight 108313 DA
Gene ID 1663
NCBI Accession NP_004390, NP_689651
UniProt Q96FC9
Pathways ER-Nucleus Signaling

Application Details

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Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References

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Background publications

Leman, Noguchi, Lee, Noguchi: "Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion." in: Journal of cell science, Vol. 123, Issue Pt 5, pp. 660-70, 2010

Farina, Shin, Kim, Bermudez, Kelman, Seo, Hurwitz: "Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1." in: The Journal of biological chemistry, Vol. 283, Issue 30, pp. 20925-36, 2008

Parish, Bean, Park, Androphy: "ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance." in: Molecular cell, Vol. 24, Issue 6, pp. 867-76, 2006

Parish, Rosa, Wang, Lahti, Doxsey, Androphy: "The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells." in: Journal of cell science, Vol. 119, Issue Pt 23, pp. 4857-65, 2006

Images

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Supplier Images
Western Blotting (WB) image for anti-DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) (AA 656-683), (Center) antibody (ABIN654411) DDX11 Antibody (Center) (ABIN654411) western blot analysis in mouse Neuro-2a cell lin...