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His Tag Antibodies

(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:
  • Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
  • Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

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  • Target: His Tag
  • Primary
Catalog no. Product Reactivity Host / Clonality Application Price & lead time
ABIN5668265
Recombinant His Tag antibody (N-Term) Primary Antibody – Mouse Monoclonal Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668257
Recombinant His Tag antibody (N-Term) Primary Antibody – Human Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN6992028
His Tag antibody Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
$577.50 View price
  • 100 μg
  • 20 μg
Delivery in 2 to 4 Business Days Shipping to: United States
ABIN7448912
His Tag antibody (FITC) Primary Antibody – Goat Polyclonal
  • IF (Immunofluorescence: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • ICC (Immunocytochemistry: Listed application)
$394.92 View price
  • 100 μg
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7468451
His Tag antibody (HRP) Primary Antibody – Mouse Monoclonal Clone GT359
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$740.00 View price
  • 100 μL
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7468450
His Tag antibody Primary Antibody – Mouse Monoclonal Clone GT161
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$740.00 View price
  • 100 μL
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7468449
His Tag antibody Primary Antibody – Mouse Monoclonal Clone GT359
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
  • ICC (Immunocytochemistry: Listed application)
$740.00 View price
  • 100 μL
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7384510
His Tag antibody Primary Antibody Various Species Mouse Monoclonal Clone 1A5
  • WB (Western Blotting: Listed application)
  • IF (Immunofluorescence: Listed application)
$107.00 View price
  • 25 μL
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN7384535
His Tag antibody Primary Antibody Various Species Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
$107.00 View price
  • 25 μL
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN7468452
His Tag antibody Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (wm) (Immunohistochemistry (Whole Mount): Listed application)
$740.00 View price
  • 100 μL
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7640269
His Tag antibody Primary Antibody Various Species Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • ICC (Immunocytochemistry: Listed application)
$360.20 View price
  • 100 μL
  • 1 mL
  • 20 μL
  • 200 μL
Delivery in 13 to 17 Business Days Shipping to: United States
ABIN7797000
His Tag antibody Primary Antibody – Mouse Monoclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
$234.22 View price
  • 100 μg
Delivery in 13 to 17 Business Days Shipping to: United States
ABIN7795837
His Tag antibody (Agarose Beads) Primary Antibody – Goat Polyclonal
  • IP (Immunoprecipitation: Listed application)
$421.85 View price
  • 100 μg
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7830310
His Tag antibody Primary Antibody Tag Goat Polyclonal
  • WB (Western Blotting: Listed application)
$577.50 View price
  • 300 μg
Delivery in 3 to 5 Business Days Shipping to: United States
ABIN7091207
His Tag antibody (Cy3) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$215.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091209
His Tag antibody (Cy5.5) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$215.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091212
His Tag antibody (AbBy Fluor® 350) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7797001
His Tag antibody Primary Antibody – Mouse Monoclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$234.22 View price
  • 100 μg
Delivery in 13 to 17 Business Days Shipping to: United States
ABIN7673382
His Tag antibody (HRP) Primary Antibody – Mouse Monoclonal Clone 1C4
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$281.16 View price
  • 100 μL
  • 500 μL
Delivery in 13 to 17 Business Days Shipping to: United States
ABIN7830311
His Tag antibody Primary Antibody Tag Goat Polyclonal
  • WB (Western Blotting: Listed application)
$577.50 View price
  • 300 μg
Delivery in 3 to 5 Business Days Shipping to: United States
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG3, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668265
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Human
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668257
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN6992028
$577.50
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 2 to 4 Business Days
Primary Antibody
  • IF (Immunofluorescence: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • ICC (Immunocytochemistry: Listed application)
Host
Goat
Clonality
Polyclonal
Conjugate
FITC
Isotype
IgG
Catalog No. ABIN7448912
$394.92
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
GT359
Conjugate
HRP
Isotype
IgG3
Catalog No. ABIN7468451
$740.00
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
GT161
Conjugate
unconjugated
Isotype
IgG2a
Catalog No. ABIN7468450
$740.00
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
  • ICC (Immunocytochemistry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
GT359
Conjugate
unconjugated
Isotype
IgG3
Catalog No. ABIN7468449
$740.00
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (Immunofluorescence: Listed application)
Reactivity
Various Species
Host
Mouse
Clonality
Monoclonal
Clone
1A5
Conjugate
unconjugated
Isotype
IgG1
Catalog No. ABIN7384510
$107.00
Plus shipping costs $50.00
25 μL
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
Reactivity
Various Species
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7384535
$107.00
Plus shipping costs $50.00
25 μL
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (wm) (Immunohistochemistry (Whole Mount): Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7468452
$740.00
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • ICC (Immunocytochemistry: Listed application)
Reactivity
Various Species
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7640269
$360.20
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 13 to 17 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
Host
Mouse
Clonality
Monoclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7797000
$234.22
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 13 to 17 Business Days
Primary Antibody
  • IP (Immunoprecipitation: Listed application)
Host
Goat
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Binding Specificity
Agarose Beads
Catalog No. ABIN7795837
$421.85
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
Reactivity
Tag
Host
Goat
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7830310
$577.50
Plus shipping costs $50.00
300 μg
Shipping to: United States
Delivery in 3 to 5 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
Cy3
Isotype
IgG
Catalog No. ABIN7091207
$215.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
Cy5.5
Isotype
IgG
Catalog No. ABIN7091209
$215.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 350
Isotype
IgG
Catalog No. ABIN7091212
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7797001
$234.22
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 13 to 17 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
1C4
Conjugate
HRP
Isotype
IgG1
Catalog No. ABIN7673382
$281.16
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 13 to 17 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
Reactivity
Tag
Host
Goat
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7830311
$577.50
Plus shipping costs $50.00
300 μg
Shipping to: United States
Delivery in 3 to 5 Business Days

Latest Publications for our His Tag Antibodies

Wang, Li, Yang, Yang, Gu, Du, Yang, Wen, Park, Huang, He: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Winans, Wucher, Nadell: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Kaufmann, Brangsch, Kader, Saatz, Mangarova, Zacharias, Kempf, Schwaar, Ponader, Adams, Möckel, Botnar, Taupitz, Mägdefessel, Traub, Hamm, Weller, Makowski: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

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