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His Tag Antibodies

(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:
  • Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
  • Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

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  • Target: His Tag
  • Primary
Catalog no. Product Reactivity Host / Clonality Application Price & lead time
ABIN6737086
His Tag antibody (Alkaline Phosphatase (AP)) Primary Antibody – Mouse Monoclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$1,254.73 View price
  • 100 μL
  • 50 μL
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN344299
His Tag antibody (C-Term) Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$785.40 View price
  • 40 μg
  • 100 μg
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN601531
His Tag antibody Primary Antibody – Goat Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IF (Immunofluorescence: Listed application)
  • IHC (Immunohistochemistry: Listed application)
$1,300.20 View price
  • 100 μg
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN595727
His Tag antibody Primary Antibody – Mouse Monoclonal
  • WB (Western Blotting: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
$1,430.73 View price
  • 200 μg
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN1968446
His Tag antibody (APC) Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$1,480.60 View price
  • 200 μL
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN1968466
His Tag antibody (PE) Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$1,480.60 View price
  • 200 μL
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN1968436
His Tag antibody (Alkaline Phosphatase (AP)) Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$1,480.60 View price
  • 200 μL
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN342052
His Tag antibody Primary Antibody – Goat Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • ICC (Immunocytochemistry: Listed application)
$788.33 View price
  • 100 μg
  • 1 mg
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN5668256
Recombinant His Tag antibody (N-Term) Primary Antibody – Human Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668258
Recombinant His Tag antibody (N-Term) Primary Antibody – Human Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668261
Recombinant His Tag antibody (N-Term) Primary Antibody – Hamster Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668262
Recombinant His Tag antibody (N-Term) Primary Antibody – Rabbit Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668264
Recombinant His Tag antibody (N-Term) Primary Antibody – Mouse Monoclonal Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668263
Recombinant His Tag antibody (N-Term) Primary Antibody – Mouse Monoclonal Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668259
Recombinant His Tag antibody (N-Term) Primary Antibody – Human Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668269
Recombinant His Tag antibody (N-Term) Primary Antibody – Rat Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668266
Recombinant His Tag antibody (N-Term) Primary Antibody – Rat Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668267
Recombinant His Tag antibody (N-Term) Primary Antibody – Rat Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668268
Recombinant His Tag antibody (N-Term) Primary Antibody – Rat Chimeric Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
ABIN5668260
Recombinant His Tag antibody (N-Term) Primary Antibody – Mouse Monoclonal Clone 3D5
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
$624.00 View price
  • 200 μg
Delivery in 7 to 13 Business Days Shipping to: United States
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Conjugate
Alkaline Phosphatase (AP)
Isotype
IgG2a
Catalog No. ABIN6737086
$1,254.73
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Binding Specificity
C-Term
Catalog No. ABIN344299
$785.40
Plus shipping costs $50.00
40 μg
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IF (Immunofluorescence: Listed application)
  • IHC (Immunohistochemistry: Listed application)
Host
Goat
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN601531
$1,300.20
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
Host
Mouse
Clonality
Monoclonal
Conjugate
unconjugated
Isotype
IgG2b
Catalog No. ABIN595727
$1,430.73
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
APC
Isotype
IgG
Catalog No. ABIN1968446
$1,480.60
Plus shipping costs $50.00
200 μL
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
PE
Isotype
IgG
Catalog No. ABIN1968466
$1,480.60
Plus shipping costs $50.00
200 μL
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
Alkaline Phosphatase (AP)
Isotype
IgG
Catalog No. ABIN1968436
$1,480.60
Plus shipping costs $50.00
200 μL
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IHC (Immunohistochemistry: Listed application)
  • ICC (Immunocytochemistry: Listed application)
Host
Goat
Clonality
Polyclonal
Conjugate
unconjugated
Catalog No. ABIN342052
$788.33
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Human
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG1, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668256
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Human
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG3, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668258
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Hamster
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668261
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Rabbit
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668262
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2c, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668264
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2b, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668263
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Human
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG4, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668259
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Rat
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2c, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668269
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Rat
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG1, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668266
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Rat
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2a, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668267
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Rat
Clonality
Chimeric
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2b, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668268
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
3D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2a, kappa
Binding Specificity
N-Term
Catalog No. ABIN5668260
$624.00
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 7 to 13 Business Days

Latest Publications for our His Tag Antibodies

Wang, Li, Yang, Yang, Gu, Du, Yang, Wen, Park, Huang, He: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Winans, Wucher, Nadell: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Kaufmann, Brangsch, Kader, Saatz, Mangarova, Zacharias, Kempf, Schwaar, Ponader, Adams, Möckel, Botnar, Taupitz, Mägdefessel, Traub, Hamm, Weller, Makowski: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

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