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His Tag Antibodies

(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:
  • Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
  • Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

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  • Target: His Tag
  • Primary
Catalog no. Product Reactivity Host / Clonality Application Price & lead time
ABIN7884701
His Tag antibody Primary Antibody – Mouse Monoclonal Clone 14-4
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • FACS (Flow Cytometry: Listed application)
  • IFA (Indirect Immunofluorescence Assay: Listed application)
$411.54 View price
  • 100 μg
Delivery in 10 to 15 Business Days Shipping to: United States
ABIN8073545
His Tag antibody Primary Antibody – Mouse Monoclonal Clone T113
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
  • ICC (Immunocytochemistry: Listed application)
$411.54 View price
  • 100 μL
  • 1 mL
  • 50 μL
  • 200 μL
Delivery in 11 to 13 Business Days Shipping to: United States
ABIN7541261
Recombinant His Tag antibody (Fab Region) Primary Antibody – Human Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541265
Recombinant His Tag antibody (Fab Region) Primary Antibody – Mouse Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541258
Recombinant His Tag antibody (Fab Region) Primary Antibody – Human Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541259
Recombinant His Tag antibody (Fab Region) Primary Antibody – Human Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541268
Recombinant His Tag antibody (Fab Region) Primary Antibody – Rat Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541269
Recombinant His Tag antibody (Fab Region) Primary Antibody – Rat Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541257
Recombinant His Tag antibody (Fab Region) Primary Antibody – Hamster Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541271
Recombinant His Tag antibody (Fab Region) Primary Antibody – Rat Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541260
Recombinant His Tag antibody (Fab Region) Primary Antibody – Human Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN7541270
Recombinant His Tag antibody (Fab Region) Primary Antibody – Rat Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
ABIN5004653
His Tag antibody (HRP) Primary Antibody – Mouse Monoclonal Clone 6E6
  • WB (Western Blotting: Listed application)
$215.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN5004652
His Tag antibody (Biotin) Primary Antibody – Mouse Monoclonal Clone 6E6
  • WB (Western Blotting: Listed application)
$196.31 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7851333
His Tag antibody (Biotin) Primary Antibody – Rabbit Monoclonal Clone DM1
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$846.12 View price
  • 100 μg
  • 10 μg
  • 500 μg
Delivery in 20 to 22 Business Days Shipping to: United States
ABIN349595
Rockland Original
His Tag antibody (HRP) Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IHC (Immunohistochemistry: Listed application)
$458.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN6720712
Rockland Original
His Tag antibody (DyLight 488) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN6720714
Rockland Original
His Tag antibody (DyLight 549) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN6720717
Rockland Original
His Tag antibody (DyLight 680) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN6720710
Rockland Original
His Tag antibody (DyLight 405) Primary Antibody – Mouse Monoclonal Clone 33D10-D2-G8
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
$490.00 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • FACS (Flow Cytometry: Listed application)
  • IFA (Indirect Immunofluorescence Assay: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
14-4
Conjugate
unconjugated
Isotype
IgG1 kappa
Catalog No. ABIN7884701
$411.54
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 10 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IP (Immunoprecipitation: Listed application)
  • IF (Immunofluorescence: Listed application)
  • ICC (Immunocytochemistry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
T113
Conjugate
unconjugated
Isotype
IgG1
Catalog No. ABIN8073545
$411.54
Plus shipping costs $50.00
100 μL (More quantities available)
Shipping to: United States
Delivery in 11 to 13 Business Days
Primary Antibody
Host
Human
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG4
Binding Specificity
Fab Region
Catalog No. ABIN7541261
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Mouse
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2
Binding Specificity
Fab Region
Catalog No. ABIN7541265
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Human
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG1
Binding Specificity
Fab Region
Catalog No. ABIN7541258
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Human
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2
Binding Specificity
Fab Region
Catalog No. ABIN7541259
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Rat
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG1
Binding Specificity
Fab Region
Catalog No. ABIN7541268
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Rat
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2a
Binding Specificity
Fab Region
Catalog No. ABIN7541269
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Hamster
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG
Binding Specificity
Fab Region
Catalog No. ABIN7541257
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Rat
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2
Binding Specificity
Fab Region
Catalog No. ABIN7541271
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Human
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG3
Binding Specificity
Fab Region
Catalog No. ABIN7541260
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
Host
Rat
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG2b
Binding Specificity
Fab Region
Catalog No. ABIN7541270
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
6E6
Conjugate
HRP
Isotype
IgG
Catalog No. ABIN5004653
$215.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
6E6
Conjugate
Biotin
Isotype
IgG
Catalog No. ABIN5004652
$196.31
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Monoclonal
Clone
DM1
Conjugate
Biotin
Isotype
IgG
Catalog No. ABIN7851333
$846.12
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 20 to 22 Business Days
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Listed application)
  • IHC (Immunohistochemistry: Listed application)
Supplier Product No.
Rockland 600-403-382
Host
Rabbit
Clonality
Polyclonal
Conjugate
HRP
Isotype
IgG
Catalog No. ABIN349595
$458.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-341-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
DyLight 488
Isotype
IgG1 kappa
Catalog No. ABIN6720712
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-342-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
DyLight 549
Isotype
IgG1 kappa
Catalog No. ABIN6720714
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-344-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
DyLight 680
Isotype
IgG1 kappa
Catalog No. ABIN6720717
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original
Primary Antibody
  • WB (Western Blotting: Validation image available)
  • ELISA (ELISA: Validation image available)
  • FM (Fluorescence Microscopy: Listed application)
  • FLISA (FLISA: Listed application)
Supplier Product No.
Rockland 200-346-382
Host
Mouse
Clonality
Monoclonal
Clone
33D10-D2-G8
Conjugate
DyLight 405
Isotype
IgG1 kappa
Catalog No. ABIN6720710
$490.00
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Rockland Original

Latest Publications for our His Tag Antibodies

Wang, Li, Yang, Yang, Gu, Du, Yang, Wen, Park, Huang, He: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Winans, Wucher, Nadell: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Kaufmann, Brangsch, Kader, Saatz, Mangarova, Zacharias, Kempf, Schwaar, Ponader, Adams, Möckel, Botnar, Taupitz, Mägdefessel, Traub, Hamm, Weller, Makowski: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

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