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His Tag Antibodies

(His Tag)
His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:
  • Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
  • Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

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  • Target: His Tag
  • Primary
Catalog no. Product Reactivity Host / Clonality Application Price & lead time
ABIN7820860
His Tag antibody (Europium W1024) Primary Antibody – Mouse Monoclonal Clone AD1-1-10
$1,176.74 View price
  • 50 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN7820862
His Tag antibody (PE) Primary Antibody – Mouse Monoclonal Clone AD1-1-10
$595.14 View price
  • 100 μg
Delivery in 1 to 3 Business Days Shipping to: United States
ABIN7091215
His Tag antibody (AbBy Fluor® 594) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091214
His Tag antibody (AbBy Fluor® 555) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091213
His Tag antibody (AbBy Fluor® 488) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091216
His Tag antibody (AbBy Fluor® 647) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091218
His Tag antibody (AbBy Fluor® 750) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091217
His Tag antibody (AbBy Fluor® 680) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$203.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091210
His Tag antibody (Cy7) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$215.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091208
His Tag antibody (Cy5) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$215.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7091211
His Tag antibody (FITC) Primary Antibody Tag Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
$215.69 View price
  • 100 μL
Delivery in 16 to 20 Business Days Shipping to: United States
ABIN7852229
His Tag antibody Primary Antibody – Chicken, Rabbit Monoclonal Clone 1B9
  • ELISA (ELISA: Listed application)
$588.01 View price
  • 100 μg
  • 10 μg
  • 500 μg
Delivery in 12 to 15 Business Days Shipping to: United States
ABIN7447741
His Tag antibody (Biotin) Primary Antibody – Rabbit Polyclonal
  • ELISA (ELISA: Listed application)
$350.77 View price
  • 100 μg
  • 1 mg
Delivery in 3 to 7 Business Days Shipping to: United States
ABIN7447739
His Tag antibody Primary Antibody – Rabbit Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IF (Immunofluorescence: Listed application)
  • IHC (Immunohistochemistry: Listed application)
$335.46 View price
  • 100 μg
  • 1 mg
Delivery in 3 to 7 Business Days Shipping to: United States
ABIN7867612
His Tag antibody Primary Antibody – Mouse Monoclonal Clone 7B8
  • ELISA (ELISA: Listed application)
$305.19 View price
  • 100 μg
Delivery in 3 to 7 Business Days Shipping to: United States
ABIN7447745
His Tag antibody (HRP) Primary Antibody – Rabbit Polyclonal
  • ELISA (ELISA: Listed application)
$365.51 View price
  • 100 μg
  • 1 mg
Delivery in 3 to 7 Business Days Shipping to: United States
ABIN2143369
His Tag antibody Primary Antibody – Chicken Polyclonal
  • WB (Western Blotting: Listed application)
$1,665.18 View price
  • 400 μL
Delivery in 9 to 12 Business Days Shipping to: United States
ABIN1832255
His Tag antibody (APC) Primary Antibody – Mouse Monoclonal Clone 11n83
  • FACS (Flow Cytometry: Listed application)
$1,413.13 View price
  • 100 tests
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN1819362
His Tag antibody (Biotin) Primary Antibody – Goat Polyclonal
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
$637.27 View price
  • 100 μg
Delivery in 11 to 15 Business Days Shipping to: United States
ABIN7541267
Recombinant His Tag antibody (Fab Region) Primary Antibody – Rabbit Monoclonal Clone 3D53D5
$1,310.40 View price
  • 200 μg
Delivery in 11 to 14 Business Days Shipping to: United States
Primary Antibody
Host
Mouse
Clonality
Monoclonal
Clone
AD1-1-10
Conjugate
Europium W1024
Catalog No. ABIN7820860
$1,176.74
Plus shipping costs $50.00
50 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Primary Antibody
Host
Mouse
Clonality
Monoclonal
Clone
AD1-1-10
Conjugate
PE
Catalog No. ABIN7820862
$595.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 1 to 3 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 594
Isotype
IgG
Catalog No. ABIN7091215
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 555
Isotype
IgG
Catalog No. ABIN7091214
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 488
Isotype
IgG
Catalog No. ABIN7091213
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 647
Isotype
IgG
Catalog No. ABIN7091216
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 750
Isotype
IgG
Catalog No. ABIN7091218
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
AbBy Fluor® 680
Isotype
IgG
Catalog No. ABIN7091217
$203.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
Cy7
Isotype
IgG
Catalog No. ABIN7091210
$215.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
Cy5
Isotype
IgG
Catalog No. ABIN7091208
$215.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • IF (p) (Immunofluorescence (Paraffin-embedded Sections): Listed application)
  • IF (cc) (Immunofluorescence (Cultured Cells): Listed application)
Reactivity
Tag
Host
Rabbit
Clonality
Polyclonal
Conjugate
FITC
Isotype
IgG
Catalog No. ABIN7091211
$215.69
Plus shipping costs $50.00
100 μL
Shipping to: United States
Delivery in 16 to 20 Business Days
Primary Antibody
  • ELISA (ELISA: Listed application)
Host
Chicken, Rabbit
Clonality
Monoclonal
Clone
1B9
Conjugate
unconjugated
Isotype
IgY
Catalog No. ABIN7852229
$588.01
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 12 to 15 Business Days
Primary Antibody
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
Biotin
Isotype
IgG
Catalog No. ABIN7447741
$350.77
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 3 to 7 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
  • IF (Immunofluorescence: Listed application)
  • IHC (Immunohistochemistry: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
unconjugated
Isotype
IgG
Catalog No. ABIN7447739
$335.46
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 3 to 7 Business Days
Primary Antibody
  • ELISA (ELISA: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
7B8
Conjugate
unconjugated
Isotype
IgG2b
Catalog No. ABIN7867612
$305.19
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 3 to 7 Business Days
Primary Antibody
  • ELISA (ELISA: Listed application)
Host
Rabbit
Clonality
Polyclonal
Conjugate
HRP
Isotype
IgG
Catalog No. ABIN7447745
$365.51
Plus shipping costs $50.00
100 μg (More quantities available)
Shipping to: United States
Delivery in 3 to 7 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
Host
Chicken
Clonality
Polyclonal
Conjugate
unconjugated
Catalog No. ABIN2143369
$1,665.18
Plus shipping costs $50.00
400 μL
Shipping to: United States
Delivery in 9 to 12 Business Days
Primary Antibody
  • FACS (Flow Cytometry: Listed application)
Host
Mouse
Clonality
Monoclonal
Clone
11n83
Conjugate
APC
Isotype
IgG1
Catalog No. ABIN1832255
$1,413.13
Plus shipping costs $50.00
100 tests
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
  • WB (Western Blotting: Listed application)
  • ELISA (ELISA: Listed application)
Host
Goat
Clonality
Polyclonal
Conjugate
Biotin
Isotype
IgG
Catalog No. ABIN1819362
$637.27
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 15 Business Days
Primary Antibody
Host
Rabbit
Clonality
Monoclonal
Clone
3D53D5
Conjugate
unconjugated
Protein Type
Recombinant Antibody
Isotype
IgG
Binding Specificity
Fab Region
Catalog No. ABIN7541267
$1,310.40
Plus shipping costs $50.00
200 μg
Shipping to: United States
Delivery in 11 to 14 Business Days

Latest Publications for our His Tag Antibodies

Wang, Li, Yang, Yang, Gu, Du, Yang, Wen, Park, Huang, He: "Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos." in: Pest management science, Vol. 80, Issue 12, pp. 6473-6482, (2024) (PubMed).

Keskin, Chen, Tsai, Du, Santos, Syu: "Reverse-Phase Protein Microarrays for Overexpressed Escherichia coli Lysates Reveal a Novel Tyrosine Kinase." in: Analytical chemistry, Vol. 96, Issue 21, pp. 8721-8729, (2024) (PubMed).

Winans, Wucher, Nadell: "Multispecies biofilm architecture determines bacterial exposure to phages." in: PLoS biology, Vol. 20, Issue 12, pp. e3001913, (2022) (PubMed).

Srivastava, Verhagen, Sasmal, Wasik, Diaz, Yu, Bensing, Khan, Khedri, Secrest, Sullam, Varki, Chen, Parrish, Varki: "Development and applications of sialoglycan-recognizing probes (SGRPs) with defined specificities: exploring the dynamic mammalian sialoglycome." in: Glycobiology, Vol. 32, Issue 12, pp. 1116-1136, (2022) (PubMed).

Khan, Sasmal, Khedri, Secrest, Verhagen, Srivastava, Varki, Chen, Yu, Beddoe, Paton, Paton, Varki: "Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits." in: The Journal of biological chemistry, Vol. 298, Issue 5, pp. 101900, (2022) (PubMed).

Minnaert, van Schie, Grootaert, Himpe, Devos, Weyts, De Keersmaecker, Braeckmans, Van Aken, Delanghe, De Smedt, Callewaert, Remaut: "Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation Spectroscopy." in: International journal of pharmaceutics, Vol. 621, pp. 121772, (2022) (PubMed).

Kaufmann, Brangsch, Kader, Saatz, Mangarova, Zacharias, Kempf, Schwaar, Ponader, Adams, Möckel, Botnar, Taupitz, Mägdefessel, Traub, Hamm, Weller, Makowski: "ADAMTS4-specific MR probe to assess aortic aneurysms in vivo using synthetic peptide libraries." in: Nature communications, Vol. 13, Issue 1, pp. 2867, (2022) (PubMed).

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." in: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Fan, Kishore, Jansen-Olliges, Wang, Stahl, Psathaki, Harre, Warnecke, Weder, Preller, Zeilinger: "Identification of a Thyroid Hormone Binding Site in Hsp90 with Implications for Its Interaction with Thyroid Hormone Receptor Beta." in: ACS omega, Vol. 7, Issue 33, pp. 28932-28945, (2022) (PubMed).

Daguer, Gonse, Shchukin, Farrera-Soler, Barluenga, Winssinger: "Dual Bcl-XL /Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy." in: Bioorganic & medicinal chemistry, Vol. 44, pp. 116282, (2021) (PubMed).

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