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|Antigen||Insulin-Like Growth Factor 2 (IGF2) Antibodies|
|Conjugate||This IGF2 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), Western Blotting (WB)
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Product Details anti-IGF2 AntibodyTarget Details IGF2 Application Details Handling Images
E.coli derived recombinant Human IGF-II.
Type of Immunogen: Cell extract
|Plasmids, Primers & others|
Target Details IGF2Product Details anti-IGF2 Antibody Application Details Handling Images back to top
|Alternative Name||IGF2 (IGF2 Antibody Abstract)|
Name/Gene ID: IGF2
Synonyms: IGF2, C11orf43, Insulin-like growth factor II, IGF-II, PP9974, Somatomedin-A
|Pathways||Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation|
Application DetailsProduct Details anti-IGF2 Antibody Target Details IGF2 Handling Images back to top
Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (3 - 5 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7415 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIGF-II (10 ng/mL), a concentration of 3.1-4.6 μg/mL of this antibody is required. ELISA: To detect hIGF-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-II (LS-C104384) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-II. Western Blot: To detect hIGF-II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Target Species of Antibody: Human
|Restrictions||For Research Use only|
HandlingProduct Details anti-IGF2 Antibody Target Details IGF2 Application Details Images back to top
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-IGF2 Antibody Target Details IGF2 Application Details Handling back to top