anti-Mudpuppy (Necturus) Vasoactive Intestinal Peptide antibody for Immunoelectron Microscopy

Recommended Vasoactive Intestinal Peptide Antibody

Antigen
Vasoactive Intestinal Peptide (Vip) Antibodies
  • PHM27
  • vip1
  • zgc:171463
  • VIP
  • vip-A
  • PHI
  • vasoactive intestinal peptide
  • vasoactive intestinal peptide L homeolog
  • vasoactive intestinal polypeptide
  • VIP
  • Vip
  • vip
  • vip.L
Reactivity
Bat, Bird (Avian), Bream (Sparus aurata), Buffalo (Bubalus), Bullfrog (Rana catesbeiana), Cat (Feline), Chicken, Columbina passerina (Common ground-dove), Cryptomys amatus (Mole rat), Dasyatis akajei (Red stingray) (Trygon akajei), Dog (Canine), Duckbill (Platypus), Fish, Fox, Frog, Goldfish, Guinea Pig, Guineafowl, Hamster, Hedgehog (Erinaceinae), Human, Lamprey, Lizard, Marine Mollusks (Liolophura), Mink, Monkey, Mouse (Murine), Mudpuppy (Necturus), Opossum (Didelphimorphia), Pig (Porcine), Pigeon, Rabbit, Raccoon (Procyon lotor), Rat (Rattus), Sheep (Ovine), Shrew, Snake, Sparrow, Squirrel, Starfish (Marthasterias), Suncus murinus, Turkey, Turtle, Worm (Spionidae), Zebra finch (Taeniopygia guttata), Zebrafish (Danio rerio)
124
59
44
21
9
8
8
6
6
5
4
4
3
2
1
Host
Rabbit
119
22
6
2
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
77
75
33
32
13
12
10
10
9
6
6
2
2
1
Options

Available images

Catalog No. ABIN617890
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General

Antigen Vasoactive Intestinal Peptide (Vip) Antibodies
Reactivity Bat, Bird (Avian), Bream (Sparus aurata), Buffalo (Bubalus), Bullfrog (Rana catesbeiana), Cat (Feline), Chicken, Columbina passerina (Common ground-dove), Cryptomys amatus (Mole rat), Dasyatis akajei (Red stingray) (Trygon akajei), Dog (Canine), Duckbill (Platypus), Fish, Fox, Frog, Goldfish, Guinea Pig, Guineafowl, Hamster, Hedgehog (Erinaceinae), Human, Lamprey, Lizard, Marine Mollusks (Liolophura), Mink, Monkey, Mouse (Murine), Mudpuppy (Necturus), Opossum (Didelphimorphia), Pig (Porcine), Pigeon, Rabbit, Raccoon (Procyon lotor), Rat (Rattus), Sheep (Ovine), Shrew, Snake, Sparrow, Squirrel, Starfish (Marthasterias), Suncus murinus, Turkey, Turtle, Worm (Spionidae), Zebra finch (Taeniopygia guttata), Zebrafish (Danio rerio)
(124), (59), (44), (21), (9), (8), (8), (6), (6), (5), (4), (4), (3), (2), (1)
Host Rabbit
(119), (22), (6), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(77), (75), (33), (32), (13), (12), (10), (10), (9), (6), (6), (2), (2), (1)
Pubmed 501 references available
Supplier

Product details

Target Details Application Details Handling References Images
Cross-Reactivity Mammalian
Characteristics The antibody has a proven strong fluorescent staining at a 1/200-1/400 dilution and a strong Biotin-Streptavidin/HRP staining at a 1/4000-1/6000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP. The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 μL of lyophilized whole serum, and 0.09 % sodium azide.
Purification Whole Serum
Immunogen Vasoactive Intestinal Peptide
Isotype IgG

Target Details

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Antigen
Alternative Name VIP (Vip Antibody Abstract)
Background Other Names:
Vasoactive intestinal polypeptide,VIP peptides
Gene ID 7432
Pathways Hormone Activity, cAMP Metabolic Process

Application Details

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Restrictions For Research Use only

Handling

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Format Liquid
Buffer Contains ≤ 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment After reconstitution, use immediately or refrigerate at 2 - 8 °C up to 2 days. For long-term storage aliquot antibody and freeze at -15 °C or lower. Avoid repeated freeze/thaw cycles.

References

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Product cited in:

Yamagata, Ogiwara, Mazaki, Yanagawa, Yamakawa: "Nav1.2 is expressed in caudal ganglionic eminence-derived disinhibitory interneurons: Mutually exclusive distributions of Nav1.1 and Nav1.2." in: Biochemical and biophysical research communications, Vol. 491, pp. 1070-1076, 2017

Strobel, Sullivan, Stratton, Sah: "Calcium signalling in medial intercalated cell dendrites and spines." in: The Journal of physiology, Vol. 595, pp. 5653-5669, 2017

Del Fiacco, Quartu, Ekström, Melis, Boi, Isola, Loy, Serra: "Effect of the neuropeptides vasoactive intestinal peptide, peptide histidine methionine and substance P on human major salivary gland secretion." in: Oral diseases, Vol. 21, Issue 2, pp. 216-23, 2016

Wu, Conlin, Morampudi, Ryz, Nasser, Bhinder, Bergstrom, Yu, Waterhouse, Buchan, Popescu, Gibson, Waschek, Vallance, Jacobson: "Vasoactive intestinal polypeptide promotes intestinal barrier homeostasis and protection against colitis in mice." in: PLoS ONE, Vol. 10, Issue 5, pp. e0125225, 2016

Kim, Soto, Kerschensteiner: "An excitatory amacrine cell detects object motion and provides feature-selective input to ganglion cells in the mouse retina." in: eLife, Vol. 4, 2016

Tuncdemir, Fishell, Batista-Brito: "miRNAs are Essential for the Survival and Maturation of Cortical Interneurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 25, Issue 7, pp. 1842-57, 2015

Ma, Yao, Fu, Yu: "Development of layer 1 neurons in the mouse neocortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 24, Issue 10, pp. 2604-18, 2014

Pohlkamp, Dávid, Cauli, Gallopin, Bouché, Karagiannis, May, Herz, Frotscher, Staiger, Bock: "Characterization and distribution of Reelin-positive interneuron subtypes in the rat barrel cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 24, Issue 11, pp. 3046-58, 2014

Hasan, Smith: "Decreased adrenoceptor stimulation in heart failure rats reduces NGF expression by cardiac parasympathetic neurons." in: Autonomic neuroscience : basic & clinical, Vol. 181, pp. 13-20, 2014

Iijima, Iijima, Witte, Scheiffele: "Neuronal cell type-specific alternative splicing is regulated by the KH domain protein SLM1." in: The Journal of cell biology, Vol. 204, Issue 3, pp. 331-42, 2014

Robertson, Croll, Smith: "The structure of the caudal wall of the zebrafish (Danio rerio) swim bladder: evidence of localized lamellar body secretion and a proximate neural plexus." in: Journal of morphology, Vol. 275, Issue 8, pp. 933-48, 2014

Tyan, Chamberland, Magnin, Camiré, Francavilla, David, Deisseroth, Topolnik: "Dendritic inhibition provided by interneuron-specific cells controls the firing rate and timing of the hippocampal feedback inhibitory circuitry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 13, pp. 4534-47, 2014

Gaykema, Nguyen, Boehret, Lambeth, Joy-Gaba, Warthen, Scott: "Characterization of excitatory and inhibitory neuron activation in the mouse medial prefrontal cortex following palatable food ingestion and food driven exploratory behavior." in: Frontiers in neuroanatomy, Vol. 8, pp. 60, 2014

Melone, Pellegrino, Nolano, Habecker, Johansson, Nathanson, Knappskog, Hahn, Boman: "Unusual Stüve-Wiedemann syndrome with complete maternal chromosome 5 isodisomy." in: Annals of clinical and translational neurology, Vol. 1, Issue 11, pp. 926-32, 2014

Perrenoud, Rossier, Geoffroy, Vitalis, Gallopin: "Diversity of GABAergic interneurons in layer VIa and VIb of mouse barrel cortex." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 23, Issue 2, pp. 423-41, 2013

Dvorackova, Macak, Brzula, Tomanova, Dokulil: "Primary neuroendocrine carcinoma of the kidney." in: Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia, Vol. 157, Issue 3, pp. 257-60, 2013

Montgomery, Whitt, Wright, Lai, Meredith: "Mis-expression of the BK K(+) channel disrupts suprachiasmatic nucleus circuit rhythmicity and alters clock-controlled behavior." in: American journal of physiology. Cell physiology, Vol. 304, Issue 4, pp. C299-311, 2013

Schuster, Dykstra, Riedl, Kitto, Honda, McIvor, Fairbanks, Vulchanova: "Visualization of spinal afferent innervation in the mouse colon by AAV8-mediated GFP expression." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 25, Issue 2, pp. e89-100, 2013

Cox, Racca: "Differential dendritic targeting of AMPA receptor subunit mRNAs in adult rat hippocampal principal neurons and interneurons." in: The Journal of comparative neurology, Vol. 521, Issue 9, pp. 1954-2007, 2013

Hioki, Okamoto, Konno, Kameda, Sohn, Kuramoto, Fujiyama, Kaneko: "Cell type-specific inhibitory inputs to dendritic and somatic compartments of parvalbumin-expressing neocortical interneuron." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 544-55, 2013

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Vasoactive Intestinal Peptide (Vip) antibody (ABIN617890) IHC image of rat cortex staining.