anti-Human CrkL antibody for Immunohistochemistry

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • CRKL
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
  • 1110025F07Rik
  • AA589403
  • AI325100
  • Crkol
  • CRK like proto-oncogene, adaptor protein
  • v-crk avian sarcoma virus CT10 oncogene homolog-like
  • CRKL
  • crkl
  • Crkl
Epitope
C-Term
33
28
17
17
15
15
14
11
10
7
6
4
4
3
3
3
2
1
1
1
1
1
1
Reactivity
Human
186
102
73
4
2
2
1
Host
Goat
141
43
23
1
Clonality
Polyclonal
Conjugate
This CrkL antibody is un-conjugated
10
9
9
8
6
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
166
88
42
25
24
23
19
7
7
5
4
3
2
2
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN184660
$ 429.84
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.12995 ABIN1867385 ICC IHC WB Rabbit IgG AA 1-303 Log in to see Polyclonal 0
13.252602 ABIN5617685 ELISA IHC WB Rabbit IgG pTyr207 Log in to see Polyclonal 0
13.252602 ABIN2138298 IF IHC ELISA WB Rabbit IgG pTyr207 Log in to see Polyclonal 0
13.252602 ABIN2138299 IF IHC ELISA WB Rabbit IgG Tyr207 Log in to see Polyclonal 0
13.12995 ABIN1531223 IF IHC ELISA WB Rabbit IgG AA 173-222, pTyr207 Log in to see Polyclonal 0
13.12995 ABIN1532241 IF IHC ELISA WB Rabbit IgG AA 173-222 Log in to see Polyclonal 0
13.12995 ABIN6270023 ELISA IHC WB Rabbit IgG pTyr251 Log in to see Polyclonal 0
13.12995 ABIN1575857 IF IHC ELISA WB Rabbit IgG Tyr207 Log in to see Polyclonal 0
13.12995 ABIN2432887 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.12995 ABIN2434506 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
13 ABIN2897233 IF/ICC IHC IP WB Rabbit AA 1-303 Log in to see Polyclonal 0
10.12995 ABIN962645 ELISA IHC IHC (p) WB Goat AA 292-303 Log in to see Polyclonal 0
10.12995 ABIN6255844 IHC WB Rabbit IgG pTyr207 Log in to see Polyclonal 0
10 ABIN2888596 ELISA IF IHC IHC (p) WB Rabbit IgG AA 173-222 Log in to see Polyclonal 0
4 ABIN2736585 IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN2562012 IHC ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
4 ABIN349260 IHC WB Rabbit IgG pTry207 Log in to see Polyclonal 0
4 ABIN349488 IHC WB Rabbit IgG Log in to see Polyclonal 0
2.479721 ABIN268650 IHC (p) IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
1 ABIN1100941 IHC WB Rabbit AA 183-232 Log in to see Polyclonal 0

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Epitope C-Term
(33), (28), (17), (17), (15), (15), (14), (11), (10), (7), (6), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(186), (102), (73), (4), (2), (2), (1)
Host Goat
(141), (43), (23), (1)
Clonality
Polyclonal
Conjugate This CrkL antibody is un-conjugated
(10), (9), (9), (8), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(166), (88), (42), (25), (24), (23), (19), (7), (7), (5), (4), (3), (2), (2), (2)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling References for anti-CrkL antibody (ABIN184660) Images
Purpose CRKL
Blocking Peptide Blocking peptide for this product available: ABIN369284
Sequence KIFDPQNPDE NE
Predicted Reactivity Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.
Plasmids, Primers & others

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling References for anti-CrkL antibody (ABIN184660) Images back to top
Antigen
Alternative Name CRKL (CRKL Antibody Abstract)
Background CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like, OTTHUMP00000198539
Gene ID 1399
NCBI Accession NP_005198
Pathways Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling References for anti-CrkL antibody (ABIN184660) Images back to top
Application Notes

DS WB Results: Approx 37 kDa band observed in lysates of cell lines K562 (calculated MW of 33.8 kDa according to NP_005198.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details References for anti-CrkL antibody (ABIN184660) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-CrkL antibody (ABIN184660)

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling Images back to top
Background publications

Singer, Hudelist, Lamm, Mueller, Handl, Kubista, Czerwenka: "Active (p)CrkL is overexpressed in human malignancies: potential role as a surrogate parameter for therapeutic tyrosine kinase inhibition." in: Oncology reports, Vol. 15, Issue 2, pp. 353-9, 2006

Images

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling References for anti-CrkL antibody (ABIN184660) back to top
Supplier Images
 image for anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (C-Term) antibody (ABIN184660) ABIN184660 (0.01µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary...