|Antigen||V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CrkL antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-CrkL AntibodyTarget Details CrkL Application Details Handling Images
|Specificity||This antibody detects CRLK at C-term.|
Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.
|Purification||Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide|
|Immunogen||Peptide from C Terminus of the protein sequence according to NP_005198.1 Genename: CRKL|
|Plasmids, Primers & others|
Target Details CrkLProduct Details anti-CrkL Antibody Application Details Handling Images back to top
|Alternative Name||CRKL (CRKL Antibody Abstract)|
|Background||Crkl is a 33 kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.Synonyms: Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like|
|Pathways||Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-CrkL Antibody Target Details CrkL Handling Images back to top
Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.01-0.03 μg/mL. Approx 37 kDa band observed in K562 and Jurkat lysates(calculated MW of 33.7 kDa according to NP_005198).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CrkL Antibody Target Details CrkL Application Details Images back to top
|Buffer||Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|