anti-Human CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Human
650
480
167
23
19
15
8
7
6
6
5
4
4
4
3
1
Host
Mouse
628
401
169
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
123
101
82
29
29
28
24
22
18
17
15
13
11
11
11
11
11
10
10
9
9
9
9
8
8
8
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
995
224
178
155
129
124
81
77
54
30
20
20
18
17
17
13
8
8
7
6
5
4
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1106134
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.728527 ABIN125690 FACS PE Mouse IgG1 Log in to see MEM-181 8
13.728527 ABIN94046 FACS FITC Mouse IgG1 Log in to see MEM-181 8
13.728527 ABIN94045 FACS Biotin Mouse IgG1 Log in to see MEM-181 8
13.728527 ABIN125691 FACS APC Mouse IgG1 Log in to see MEM-181 8
13.728527 ABIN94044 FACS Mouse IgG1 Log in to see MEM-181 8
13.728527 ABIN4896455 FACS PE Mouse IgG2a AA 22-213 Log in to see 24212 5
13.728527 ABIN1027672 FACS PerCP Mouse IgG1 Log in to see MEM-181 4
13.728527 ABIN2749120 FACS PE-DyLight 594 Mouse IgG1 Log in to see MEM-181 4
13.728527 ABIN264241 FACS PE Mouse IgG1 Log in to see 1TYV 4
13.728527 ABIN1106144 FACS Biotin Mouse IgG1 Log in to see B-B10 1
13.728527 ABIN1106146 FACS FITC Mouse IgG1 Log in to see B-F2 1
13.728527 ABIN1106135 Func IHC (fro) FACS Mouse IgG1 Log in to see B-F2 1
13.728527 ABIN5668051 BR FACS IF IHC WB Rabbit IgG, kappa Log in to see Chimeric 0
13.728527 ABIN1106137 IHC (fro) FACS Mouse IgG1 Log in to see B-F2 0
13.728527 ABIN1106136 FACS Mouse IgG1 Log in to see B-G3 1
13.728527 ABIN4896451 FACS APC Mouse IgG2a AA 22-213 Log in to see 24212 0
13.728527 ABIN4896453 FACS Alexa Fluor 488 Mouse IgG2a AA 22-213 Log in to see 24212 0
13.728527 ABIN2851681 FACS IF PE Mouse IgG2a Log in to see 0
13.728527 ABIN2851731 FACS IF PE-Cy5 Mouse IgG2a Log in to see 0
13.728527 ABIN2851747 FACS IF Biotin Mouse IgG2a Log in to see 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(650), (480), (167), (23), (19), (15), (8), (7), (6), (6), (5), (4), (4), (4), (3), (1)
Host Mouse
(628), (401), (169), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (123), (101), (82), (29), (29), (28), (24), (22), (18), (17), (15), (13), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (8), (8), (8), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS)
(995), (224), (178), (155), (129), (124), (81), (77), (54), (30), (20), (20), (18), (17), (17), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 18 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1106134) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ion Exchange Chromatography.
Sterility 0.22 μm filtered
Immunogen PHA activated T cells. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells
Clone B-B10
Isotype IgG1
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1106134) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background IL-2R (T-cell growth factor receptor) consists of a chain of 55 kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2RA is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 Molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 3559
NCBI Accession NP_000408
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1106134) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1106134) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate-buffered saline without Carrier and preservatives. This prodcut is sterile-filtered through 0.22 μm and treated to remove Endotoxins.
Preservative Without preservative
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-CD25 antibody (ABIN1106134)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Regeer, Markovich: "A dileucine motif targets the sulfate anion transporter sat-1 to the basolateral membrane in renal cell lines." in: American journal of physiology. Cell physiology, Vol. 287, Issue 2, pp. C365-72, 2004

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, 2001

Groux, Bigler, de Vries, Roncarolo: "Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3188-93, 1998

Nevill, Fung, Shepherd, Horsman, Nantel, Klingemann, Forrest, Toze, Sutherland, Hogge, Naiman, Le, Brockington, Barnett: "Cytogenetic abnormalities in primary myelodysplastic syndrome are highly predictive of outcome after allogeneic bone marrow transplantation." in: Blood, Vol. 92, Issue 6, pp. 1910-7, 1998

Ozsahin, von Planta, Müller, Steinert, Nadal, Lauener, Tuchschmid, Willi, Ozsahin, Crompton, Seger: "Successful treatment of invasive aspergillosis in chronic granulomatous disease by bone marrow transplantation, granulocyte colony-stimulating factor-mobilized granulocytes, and liposomal amphotericin-B." in: Blood, Vol. 92, Issue 8, pp. 2719-24, 1998

Zambello, Facco, Trentin, Sancetta, Tassinari, Perin, Milani, Pizzolo, Rodeghiero, Agostini, Meazza, Ferrini, Semenzato: "Interleukin-15 triggers the proliferation and cytotoxicity of granular lymphocytes in patients with lymphoproliferative disease of granular lymphocytes." in: Blood, Vol. 89, Issue 1, pp. 201-11, 1997

Mousson, Tanter, Racadot, Wijdenes, Chalopin, Chevet, Hervé, Rifle: "CD4 and CD25 monoclonal antibody cocktail in kidney transplant rejection prophylaxis: clinical results of a pilot study." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1727-8, 1995

Racadot, Milpied, Bordigoni, Cahn, Plouvier, Lioure, Lutz, Wijdenes, Herve: "Sequential use of three monoclonal antibodies in corticosteroid-resistant acute GVHD: a multicentric pilot study including 15 patients." in: Bone marrow transplantation, Vol. 15, Issue 5, pp. 669-77, 1995

Racadot, Mousson, Tanter, Wijdenes, Rifle, Herve: "Outcome of lymphocyte subsets and cytokines during combined CD4/anti-IL-2R monoclonal antibody therapy in kidney transplantation." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1672-3, 1995

Herbelin, Stephan, Donadieu, Le Deist, Racadot, Wijdenes, Fischer: "Treatment of steroid-resistant acute graft-versus-host disease with an anti-IL-2-receptor monoclonal antibody (BT 563) in children who received T cell-depleted, partially matched, related bone marrow transplants." in: Bone marrow transplantation, Vol. 13, Issue 5, pp. 563-9, 1994

Nakatani, Lone, Yamakawa, Kanaoka, Gomi, Wijdenes, Noguchi: "Humanization of mouse anti-human IL-2 receptor antibody B-B10." in: Protein engineering, Vol. 7, Issue 3, pp. 435-43, 1994

Tazzari, Bolognesi, De Totero, Pileri, Conte, Wijdenes, Hervé, Soria, Stirpe, Gobbi: "B-B10 (anti-CD25)-saporin immunotoxin--a possible tool in graft-versus-host disease treatment." in: Transplantation, Vol. 54, Issue 2, pp. 351-6, 1992

Engert, Martin, Amlot, Wijdenes, Diehl, Thorpe: "Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 3, pp. 450-6, 1991

Heyll, Aul, Söhngen, Meckenstock, Arning, Wurm, Schmitt, Schneider: "[Therapy of multiple myeloma by allogeneic bone marrow transplantation]." in: Deutsche medizinische Wochenschrift (1946), Vol. 116, Issue 22, pp. 852-6, 1991

Tiberghien, Racadot, Lioure, Delain, Girard, Wijdenes, Plouvier, Flesch, Cahn, Herve: "Soluble CD8, IL-2 receptor, and tumor necrosis factor-alpha levels in steroid-resistant acute graft-versus-host disease. Relation with subsequent response to anti-IL-2 receptor monoclonal antibody treatment." in: Transplantation, Vol. 52, Issue 3, pp. 475-80, 1991

Wijdenes, Beliard, Muot, Herve, Peters: "A semi-pharmaceutical approach for the preparation of an anti-IL2 receptor monoclonal antibody in the treatment of acute GvHD in a multicentric study." in: Developments in biological standardization, Vol. 71, pp. 103-11, 1990

Hervé, Wijdenes, Bergerat, Bordigoni, Milpied, Cahn, Clément, Béliard, Morel-Fourrier, Racadot: "Treatment of corticosteroid resistant acute graft-versus-host disease by in vivo administration of anti-interleukin-2 receptor monoclonal antibody (B-B10)" in: Blood, Vol. 75, Issue 4, pp. 1017-23, 1990

Hervé, Wijdenes, Bergerat, Milpied, Gaud, Bordigoni: "Treatment of acute graft-versus-host disease with monoclonal antibody to IL-2 receptor." in: Lancet, Vol. 2, Issue 8619, pp. 1072-3, 1988

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1106134) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN1106134) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody