anti-Human CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Human
625
452
145
22
16
8
7
6
6
5
4
4
1
1
1
1
Host
Mouse
593
385
164
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
123
95
83
29
26
25
22
20
15
14
13
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
940
201
163
152
117
109
79
75
48
20
20
18
17
17
16
13
8
8
7
6
5
4
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN1383666
$ 324.85
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.451893 ABIN1027672 FACS PerCP Mouse IgG1 Log in to see MEM-181 4
26.951893 ABIN5668051 BR FACS IF IHC WB Rabbit IgG, kappa Log in to see Chimeric 0
25.451893 ABIN94046 FACS FITC Mouse IgG1 Log in to see MEM-181 8
25.451893 ABIN125691 FACS APC Mouse IgG1 Log in to see MEM-181 8
25.451893 ABIN125690 FACS PE Mouse IgG1 Log in to see MEM-181 8
25.451893 ABIN94045 FACS Biotin Mouse IgG1 Log in to see MEM-181 8
25.451893 ABIN94044 FACS Mouse IgG1 Log in to see MEM-181 8
25.451893 ABIN2749120 FACS PE-DyLight 594 Mouse IgG1 Log in to see MEM-181 4
20.951893 ABIN94049 FACS IP Mouse IgM Log in to see MEM-140 3
20.951893 ABIN2144371 FACS PE-Cy5 Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144297 FACS Biotin Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144369 FACS PE-Cy5 Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144295 FACS Biotin Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144299 FACS Biotin Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144301 FACS Biotin Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144548 FACS FITC Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144546 FACS FITC Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144835 FACS PE Mouse IgG2a Log in to see 4A7G7B6 0
20.951893 ABIN2144833 FACS PE Mouse IgG2a Log in to see 4A7G7B6 0
20.07959 ABIN1383667 FACS Biotin Mouse IgG1 Log in to see B-B10 1

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(625), (452), (145), (22), (16), (8), (7), (6), (6), (5), (4), (4), (1), (1), (1), (1)
Host Mouse
(593), (385), (164), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (123), (95), (83), (29), (26), (25), (22), (20), (15), (14), (13), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(940), (201), (163), (152), (117), (109), (79), (75), (48), (20), (20), (18), (17), (17), (16), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 18 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1383666) Images
Specificity Recognises the Interleukin 2 Receptor a chain (IL-2 Ra), a 55 kDa protein
Characteristics PHA activated T cells. Carrier and preservative free
Purification Ion exchange chromatography
Sterility 0.22 μm filtered
Clone B-B10
Isotype IgG1
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1383666) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background Myeloma X63/AG.8653 x Balb/c spleen cells
Molecular Weight 55 kDa
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1383666) Images back to top
Application Notes Suitable for flow cytometry. Inhibits Mixed Lymphocyte Culture. Recognises only membrane bound human IL-2 Ra
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1383666) Images back to top
Format Liquid
Concentration 200µg/200µl
Buffer Phosphate-buffered saline.
Preservative Azide free
Storage 4 °C
Storage Comment Declared stable after 7 days at room temperature. Protect from light. Do not freeze.
Expiry Date 1 week

References for anti-CD25 antibody (ABIN1383666)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Regeer, Markovich: "A dileucine motif targets the sulfate anion transporter sat-1 to the basolateral membrane in renal cell lines." in: American journal of physiology. Cell physiology, Vol. 287, Issue 2, pp. C365-72, 2004

Groux, Bigler, de Vries, Roncarolo: "Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3188-93, 1998

Nevill, Fung, Shepherd, Horsman, Nantel, Klingemann, Forrest, Toze, Sutherland, Hogge, Naiman, Le, Brockington, Barnett: "Cytogenetic abnormalities in primary myelodysplastic syndrome are highly predictive of outcome after allogeneic bone marrow transplantation." in: Blood, Vol. 92, Issue 6, pp. 1910-7, 1998

Ozsahin, von Planta, Müller, Steinert, Nadal, Lauener, Tuchschmid, Willi, Ozsahin, Crompton, Seger: "Successful treatment of invasive aspergillosis in chronic granulomatous disease by bone marrow transplantation, granulocyte colony-stimulating factor-mobilized granulocytes, and liposomal amphotericin-B." in: Blood, Vol. 92, Issue 8, pp. 2719-24, 1998

Agostini, Trentin, Sancetta, Facco, Tassinari, Cerutti, Bortolin, Milani, Siviero, Zambello, Semenzato: "Interleukin-15 triggers activation and growth of the CD8 T-cell pool in extravascular tissues of patients with acquired immunodeficiency syndrome." in: Blood, Vol. 90, Issue 3, pp. 1115-23, 1997

Racadot, Mousson, Tanter, Wijdenes, Rifle, Herve: "Outcome of lymphocyte subsets and cytokines during combined CD4/anti-IL-2R monoclonal antibody therapy in kidney transplantation." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1672-3, 1995

Racadot, Milpied, Bordigoni, Cahn, Plouvier, Lioure, Lutz, Wijdenes, Herve: "Sequential use of three monoclonal antibodies in corticosteroid-resistant acute GVHD: a multicentric pilot study including 15 patients." in: Bone marrow transplantation, Vol. 15, Issue 5, pp. 669-77, 1995

Mousson, Tanter, Racadot, Wijdenes, Chalopin, Chevet, Hervé, Rifle: "CD4 and CD25 monoclonal antibody cocktail in kidney transplant rejection prophylaxis: clinical results of a pilot study." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1727-8, 1995

Herbelin, Stephan, Donadieu, Le Deist, Racadot, Wijdenes, Fischer: "Treatment of steroid-resistant acute graft-versus-host disease with an anti-IL-2-receptor monoclonal antibody (BT 563) in children who received T cell-depleted, partially matched, related bone marrow transplants." in: Bone marrow transplantation, Vol. 13, Issue 5, pp. 563-9, 1994

Nakatani, Lone, Yamakawa, Kanaoka, Gomi, Wijdenes, Noguchi: "Humanization of mouse anti-human IL-2 receptor antibody B-B10." in: Protein engineering, Vol. 7, Issue 3, pp. 435-43, 1994

Tazzari, Bolognesi, De Totero, Pileri, Conte, Wijdenes, Hervé, Soria, Stirpe, Gobbi: "B-B10 (anti-CD25)-saporin immunotoxin--a possible tool in graft-versus-host disease treatment." in: Transplantation, Vol. 54, Issue 2, pp. 351-6, 1992

Heyll, Aul, Söhngen, Meckenstock, Arning, Wurm, Schmitt, Schneider: "[Therapy of multiple myeloma by allogeneic bone marrow transplantation]." in: Deutsche medizinische Wochenschrift (1946), Vol. 116, Issue 22, pp. 852-6, 1991

Engert, Martin, Amlot, Wijdenes, Diehl, Thorpe: "Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 3, pp. 450-6, 1991

Tiberghien, Racadot, Lioure, Delain, Girard, Wijdenes, Plouvier, Flesch, Cahn, Herve: "Soluble CD8, IL-2 receptor, and tumor necrosis factor-alpha levels in steroid-resistant acute graft-versus-host disease. Relation with subsequent response to anti-IL-2 receptor monoclonal antibody treatment." in: Transplantation, Vol. 52, Issue 3, pp. 475-80, 1991

Wijdenes, Beliard, Muot, Herve, Peters: "A semi-pharmaceutical approach for the preparation of an anti-IL2 receptor monoclonal antibody in the treatment of acute GvHD in a multicentric study." in: Developments in biological standardization, Vol. 71, pp. 103-11, 1990

Hervé, Wijdenes, Bergerat, Bordigoni, Milpied, Cahn, Clément, Béliard, Morel-Fourrier, Racadot: "Treatment of corticosteroid resistant acute graft-versus-host disease by in vivo administration of anti-interleukin-2 receptor monoclonal antibody (B-B10)" in: Blood, Vol. 75, Issue 4, pp. 1017-23, 1990

Hervé, Wijdenes, Bergerat, Milpied, Gaud, Bordigoni: "Treatment of acute graft-versus-host disease with monoclonal antibody to IL-2 receptor." in: Lancet, Vol. 2, Issue 8619, pp. 1072-3, 1988

Background publications

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, 2001

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1383666) back to top
Supplier Images
 image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN1383666) A typical staining pattern with the B-B10 monoclonal antibody of PHA activated lympho...