anti-Human CD25 antibody for Functional Studies

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Human
589
482
165
22
19
16
9
8
6
6
5
4
4
4
3
1
Host
Mouse
584
407
151
7
6
3
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
117
95
79
30
29
29
24
23
18
16
14
12
12
12
12
12
11
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
937
191
171
151
128
89
88
83
60
30
22
21
19
17
13
12
9
8
7
6
6
5
3
2
1
1
1
1
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Catalog No. ABIN1106134
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106135 Func IHC (fro) FACS Mouse IgG1 Log in to see B-F2 1
1 ABIN1721247 Func FACS IHC Biotin Mouse IgG1 Log in to see B-B10 0
1 ABIN1722531 Func FACS IHC ELISA Mouse IgG1 Log in to see B-F2 0
1 ABIN181143 EIA Func IHC (fro) WB Mouse IgG2a Log in to see 0
1 ABIN1722530 Func FACS IHC Mouse IgG1 Log in to see B-B10 0
1 ABIN263858 Func FACS Mouse IgG1 Log in to see NR-72 0
1 ABIN1177330 Func FACS Mouse IgG1, kappa Log in to see M-A251 0
1 ABIN3025893 Func FACS IF Mouse IgG1, kappa Log in to see 143-13 0
1 ABIN3025895 Func FACS IF Mouse IgG2a, kappa Log in to see IL2RA-423 0
1 ABIN3025894 Func FACS IF Mouse IgG1, kappa Log in to see 143-13 0
1 ABIN3025896 Func FACS IF Mouse IgG2a, kappa Log in to see IL2RA-423 0
1 ABIN2136292 Func FACS IHC Mouse IgG1 kappa Log in to see BC96 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Human
(589), (482), (165), (22), (19), (16), (9), (8), (6), (6), (5), (4), (4), (4), (3), (1)
Host Mouse
(584), (407), (151), (7), (6), (3), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (117), (95), (79), (30), (29), (29), (24), (23), (18), (16), (14), (12), (12), (12), (12), (12), (11), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS)
(937), (191), (171), (151), (128), (89), (88), (83), (60), (30), (22), (21), (19), (17), (13), (12), (9), (8), (7), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 18 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1106134) Images
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ion Exchange Chromatography.
Sterility 0.22 μm filtered
Immunogen PHA activated T cells. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells
Clone B-B10
Isotype IgG1

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1106134) Images back to top
Antigen
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background IL-2R (T-cell growth factor receptor) consists of a chain of 55 kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2RA is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 Molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 3559
NCBI Accession NP_000408
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1106134) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1106134) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate-buffered saline without Carrier and preservatives. This prodcut is sterile-filtered through 0.22 μm and treated to remove Endotoxins.
Preservative Without preservative
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-CD25 antibody (ABIN1106134)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Regeer, Markovich: "A dileucine motif targets the sulfate anion transporter sat-1 to the basolateral membrane in renal cell lines." in: American journal of physiology. Cell physiology, Vol. 287, Issue 2, pp. C365-72, 2004

Oppmann, Lesley, Blom, Timans, Xu, Hunte, Vega, Yu, Wang, Singh, Zonin, Vaisberg, Churakova, Liu, Gorman, Wagner, Zurawski, Liu, Abrams, Moore, Rennick, de Waal-Malefyt, Hannum, Bazan, Kastelein: "Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12." in: Immunity, Vol. 13, Issue 5, pp. 715-25, 2001

Groux, Bigler, de Vries, Roncarolo: "Inhibitory and stimulatory effects of IL-10 on human CD8+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 7, pp. 3188-93, 1998

Nevill, Fung, Shepherd, Horsman, Nantel, Klingemann, Forrest, Toze, Sutherland, Hogge, Naiman, Le, Brockington, Barnett: "Cytogenetic abnormalities in primary myelodysplastic syndrome are highly predictive of outcome after allogeneic bone marrow transplantation." in: Blood, Vol. 92, Issue 6, pp. 1910-7, 1998

Ozsahin, von Planta, Müller, Steinert, Nadal, Lauener, Tuchschmid, Willi, Ozsahin, Crompton, Seger: "Successful treatment of invasive aspergillosis in chronic granulomatous disease by bone marrow transplantation, granulocyte colony-stimulating factor-mobilized granulocytes, and liposomal amphotericin-B." in: Blood, Vol. 92, Issue 8, pp. 2719-24, 1998

Zambello, Facco, Trentin, Sancetta, Tassinari, Perin, Milani, Pizzolo, Rodeghiero, Agostini, Meazza, Ferrini, Semenzato: "Interleukin-15 triggers the proliferation and cytotoxicity of granular lymphocytes in patients with lymphoproliferative disease of granular lymphocytes." in: Blood, Vol. 89, Issue 1, pp. 201-11, 1997

Mousson, Tanter, Racadot, Wijdenes, Chalopin, Chevet, Hervé, Rifle: "CD4 and CD25 monoclonal antibody cocktail in kidney transplant rejection prophylaxis: clinical results of a pilot study." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1727-8, 1995

Racadot, Milpied, Bordigoni, Cahn, Plouvier, Lioure, Lutz, Wijdenes, Herve: "Sequential use of three monoclonal antibodies in corticosteroid-resistant acute GVHD: a multicentric pilot study including 15 patients." in: Bone marrow transplantation, Vol. 15, Issue 5, pp. 669-77, 1995

Racadot, Mousson, Tanter, Wijdenes, Rifle, Herve: "Outcome of lymphocyte subsets and cytokines during combined CD4/anti-IL-2R monoclonal antibody therapy in kidney transplantation." in: Transplantation proceedings, Vol. 27, Issue 2, pp. 1672-3, 1995

Herbelin, Stephan, Donadieu, Le Deist, Racadot, Wijdenes, Fischer: "Treatment of steroid-resistant acute graft-versus-host disease with an anti-IL-2-receptor monoclonal antibody (BT 563) in children who received T cell-depleted, partially matched, related bone marrow transplants." in: Bone marrow transplantation, Vol. 13, Issue 5, pp. 563-9, 1994

Nakatani, Lone, Yamakawa, Kanaoka, Gomi, Wijdenes, Noguchi: "Humanization of mouse anti-human IL-2 receptor antibody B-B10." in: Protein engineering, Vol. 7, Issue 3, pp. 435-43, 1994

Tazzari, Bolognesi, De Totero, Pileri, Conte, Wijdenes, Hervé, Soria, Stirpe, Gobbi: "B-B10 (anti-CD25)-saporin immunotoxin--a possible tool in graft-versus-host disease treatment." in: Transplantation, Vol. 54, Issue 2, pp. 351-6, 1992

Engert, Martin, Amlot, Wijdenes, Diehl, Thorpe: "Immunotoxins constructed with anti-CD25 monoclonal antibodies and deglycosylated ricin A-chain have potent anti-tumour effects against human Hodgkin cells in vitro and solid Hodgkin tumours in mice." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 3, pp. 450-6, 1991

Heyll, Aul, Söhngen, Meckenstock, Arning, Wurm, Schmitt, Schneider: "[Therapy of multiple myeloma by allogeneic bone marrow transplantation]." in: Deutsche medizinische Wochenschrift (1946), Vol. 116, Issue 22, pp. 852-6, 1991

Tiberghien, Racadot, Lioure, Delain, Girard, Wijdenes, Plouvier, Flesch, Cahn, Herve: "Soluble CD8, IL-2 receptor, and tumor necrosis factor-alpha levels in steroid-resistant acute graft-versus-host disease. Relation with subsequent response to anti-IL-2 receptor monoclonal antibody treatment." in: Transplantation, Vol. 52, Issue 3, pp. 475-80, 1991

Wijdenes, Beliard, Muot, Herve, Peters: "A semi-pharmaceutical approach for the preparation of an anti-IL2 receptor monoclonal antibody in the treatment of acute GvHD in a multicentric study." in: Developments in biological standardization, Vol. 71, pp. 103-11, 1990

Hervé, Wijdenes, Bergerat, Bordigoni, Milpied, Cahn, Clément, Béliard, Morel-Fourrier, Racadot: "Treatment of corticosteroid resistant acute graft-versus-host disease by in vivo administration of anti-interleukin-2 receptor monoclonal antibody (B-B10)" in: Blood, Vol. 75, Issue 4, pp. 1017-23, 1990

Hervé, Wijdenes, Bergerat, Milpied, Gaud, Bordigoni: "Treatment of acute graft-versus-host disease with monoclonal antibody to IL-2 receptor." in: Lancet, Vol. 2, Issue 8619, pp. 1072-3, 1988

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1106134) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN1106134) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody