anti-Mouse (Murine) CD25 antibody for Flow Cytometry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Mouse (Murine)
590
481
165
22
19
16
9
8
6
6
5
4
4
4
3
1
Host
Rat
585
406
151
7
6
3
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
131
117
95
79
30
29
29
24
23
18
16
14
12
12
12
12
12
11
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
937
191
171
151
128
89
88
83
61
30
22
21
19
17
13
12
9
8
7
6
6
5
3
2
1
1
1
1
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Catalog No. ABIN2665963
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.93816 ABIN4896465 FACS APC Rat IgG2a AA 22-236 Log in to see 280406 1
13.93816 ABIN4896466 FACS APC Rat IgG2a AA 22-236 Log in to see 280406 1
13.93816 ABIN2659693 FACS PE/Dazzle™ 594 Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2662234 FACS Pacific Blue Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2661005 FACS Biotin Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2657371 FACS Alexa Fluor 488 Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2657710 CyTOF FACS Alexa Fluor 647 Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2658857 FACS APC Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2658949 FACS IP IHC PE-Cy5 Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN4260058 CyTOF FACS in vivo Rat IgG Mix lambda Log in to see PC61 1
13.93816 ABIN2664438 FACS Rat IgG1 lambda Log in to see PC61 12
13.93816 ABIN2658552 FACS IF IHC APC Rat IgG2b kappa Log in to see 3C7 8
13.93816 ABIN951247 IHC (fro) FACS IP Biotin Rat IgG1 Log in to see PC61-5-3 0
13.93816 ABIN951248 FACS FITC Rat IgG1 Log in to see PC61-5-3 0
13.93816 ABIN370886 FACS PE Rat IgM Log in to see 7D4 0
13.93816 ABIN4896471 FACS PE Rat IgG2a AA 22-236 Log in to see 280406 0
13.93816 ABIN4896467 FACS PerCP Rat IgG2a AA 22-236 Log in to see 280406 0
13.93816 ABIN4896468 FACS PerCP Rat IgG2a AA 22-236 Log in to see 280406 0
13.93816 ABIN4896470 FACS Alexa Fluor 488 Rat IgG2a AA 22-236 Log in to see 280406 0
13.93816 ABIN4896472 FACS PE Rat IgG2a AA 22-236 Log in to see 280406 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(590), (481), (165), (22), (19), (16), (9), (8), (6), (6), (5), (4), (4), (4), (3), (1)
Host Rat
(585), (406), (151), (7), (6), (3), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(131), (117), (95), (79), (30), (29), (29), (24), (23), (18), (16), (14), (12), (12), (12), (12), (12), (11), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(937), (191), (171), (151), (128), (89), (88), (83), (61), (30), (22), (21), (19), (17), (13), (12), (9), (8), (7), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2665963) Images
Brand Ultra-LEAF™
Purification The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level

Endotoxin level is <0.01 EU/μg of the protein (<0.001 ng/μg of the protein) as determined by the LAL test.

Clone PC61
Isotype IgG1 lambda

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN2665963) Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN2665963) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN2665963) Images back to top
Concentration 1.0 mg/mL
Preservative Azide free
Handling Advice This Ultra-LEAF™ solution contains no preservative, handle under aseptic conditions
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD25 antibody (ABIN2665963)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

León-Ponte, Ahern, OConnell: "Serotonin provides an accessory signal to enhance T-cell activation by signaling through the 5-HT7 receptor." in: Blood, Vol. 109, Issue 8, pp. 3139-46, 2007

Lei, Scott: "Induction of tolerance to factor VIII inhibitors by gene therapy with immunodominant A2 and C2 domains presented by B cells as Ig fusion proteins." in: Blood, Vol. 105, Issue 12, pp. 4865-70, 2005

Pasare, Medzhitov: "Toll-dependent control mechanisms of CD4 T cell activation." in: Immunity, Vol. 21, Issue 5, pp. 733-41, 2004

Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, 2000

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, 2000

Onizuka, Tawara, Shimizu, Sakaguchi, Fujita, Nakayama: "Tumor rejection by in vivo administration of anti-CD25 (interleukin-2 receptor alpha) monoclonal antibody." in: Cancer research, Vol. 59, Issue 13, pp. 3128-33, 1999

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, 1991

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, 1987

Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, 1985

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, 1985

Ceredig, Lowenthal, Nabholz, MacDonald: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, 1985

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN2665963) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN2665963) anti-Interleukin 2 Receptor, alpha (IL2RA) antibody