anti-Mouse (Murine) CD25 antibody for Immunofluorescence

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Mouse (Murine)
631
441
144
22
16
8
6
6
6
5
4
4
1
1
1
1
Host
Rat
593
372
167
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is conjugated to APC
129
119
93
81
26
25
25
21
20
14
13
12
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
933
196
166
142
117
110
76
74
40
20
20
18
17
17
16
13
8
8
5
5
4
2
2
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN1112441
$ 578.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.32936 ABIN1112442 FACS IF PerCP Rat IgG1 Log in to see PC61 0
12.32936 ABIN3044664 FACS IF Rat IgG1 Log in to see PC61 0
9.876598 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12 0
7 ABIN6266235 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN2658552 FACS IF IHC APC Rat IgG2b kappa Log in to see 3C7 8
1 ABIN2658553 FACS IF IHC APC Rat IgG2b kappa Log in to see 3C7 8
-2.3894138 ABIN153451 ICC IHC (fro) IF IHC (p) IHC Mouse IgG1 kappa Log in to see IL2R-1 5
-12.889414 ABIN4291069 BP CyTOF FACS IF IHC Neut WB Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab 0
-12.889414 ABIN4290998 ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see IL2R-1 0
-12.889414 ABIN5945614 FACS IF IHC Janelia Fluor® 646 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab 0
-12.889414 ABIN5945613 FACS IF IHC Janelia Fluor® 549 Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Mouse (Murine)
(631), (441), (144), (22), (16), (8), (6), (6), (6), (5), (4), (4), (1), (1), (1), (1)
Host Rat
(593), (372), (167), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is conjugated to APC
(129), (119), (93), (81), (26), (25), (25), (21), (20), (14), (13), (12), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF)
(933), (196), (166), (142), (117), (110), (76), (74), (40), (20), (20), (18), (17), (17), (16), (13), (8), (8), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Characteristics Monoclonal Rat Anti-Mouse CD25 APC is recommended for use in flow cytometry for identification of the Mouse CD25 molecule, present on activated T and B cells.
Immunogen IL-2-dependent cytolytic mouse T-cell clone B6.1
Clone PC61
Isotype IgG1
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD25 (IL2RA Antibody Abstract)
Background CD25 is a 55 kDa glycoprotein, also known as the low affinity IL-2Ralpha, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. association with CD122 (IL-2Rbeta) and CD132 (common gamma chain), CD25 forms the high affinity signaling IL-2 receptor. The PC61 antibody is able to block IL-12 binding to its receptor and inhibit IL-2 stimulated cell growth.
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is less or equal to 1.0 µgper million cells in 100 µL volume.
Comment

Allophycocyanin (APC), Abs/Em: 651/662 nm.

Reagent Preparation 1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add CD25 APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Format Liquid
Buffer The conjugate is provided in liquid form in buffer containing PBS, 1% BSA and 0.09 % Sodium azide, pH 7,4.
Preservative Sodium azide
Precaution of Use 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
Storage 4 °C