anti-Rhesus Monkey CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Reactivity
Cynomolgus, Human, Rhesus Monkey
625
452
145
22
16
8
7
6
6
4
4
3
1
1
1
1
Host
Human
594
385
164
11
6
4
3
2
Antibody Type
Recombinant Antibody
Clonality
Chimeric
Conjugate
This CD25 antibody is un-conjugated
130
123
95
83
29
26
25
22
20
15
14
13
11
10
10
10
10
10
9
9
9
9
9
6
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
940
200
162
152
116
109
79
75
48
20
20
18
17
17
16
13
8
7
7
6
5
4
2
2
1
1
1
1
1
1
1
Options
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Available images

Catalog No. ABIN5668050
$ 345.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5668051 BR FACS IF IHC WB Rabbit IgG, kappa Log in to see Chimeric 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Reactivity Cynomolgus, Human, Rhesus Monkey
(625), (452), (145), (22), (16), (8), (7), (6), (6), (4), (4), (3), (1), (1), (1), (1)
Host Human
(594), (385), (164), (11), (6), (4), (3), (2)
Clonality (Clone)
Chimeric   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (123), (95), (83), (29), (26), (25), (22), (20), (15), (14), (13), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(940), (200), (162), (152), (116), (109), (79), (75), (48), (20), (20), (18), (17), (17), (16), (13), (8), (7), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
Characteristics Human IgG1 based on the original Human IgG1
Purification Purified antibody.
Purity > 98 % as determined by SDS-PAGE
Endotoxin Level Endotoxin is < 1.0 EU/mg as determined by the LAL method
Immunogen Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Clone Basiliximab
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IL-2R alpha (CD25) (IL2RA Antibody Abstract)
Background Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD25.
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1? constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.
Comment

NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Buffer PBS with 0.02 % Proclin 300.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN5668050) Immunohistochemical staining of rat spleens using anti-CD25 antibody Formalin fixed ...
Immunofluorescence (IF) image for anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (ABIN5668050) Immunofluorescent staining of rat thymus using anti-CD25 antibody Formaldehyde-fixe...