anti-Mouse (Murine) FYN antibody for Immunoprecipitation

Recommended FYN Antibody (supplied by: Log in to see )

Antigen
FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
  • im:6908851
  • si:dkey-119m7.5
  • zgc:109996
  • c-fyn
  • cfyn-a
  • SLK
  • SYN
  • p59-FYN
  • PKC
  • fyn
  • zgc:86720
  • AI448320
  • AW552119
  • FYN proto-oncogene, Src family tyrosine kinase
  • FYN proto-oncogene, Src family tyrosine kinase S homeolog
  • FYN proto-oncogene, Src family tyrosine kinase b
  • FYN proto-oncogene, Src family tyrosine kinase L homeolog
  • FYN proto-oncogene, Src family tyrosine kinase a
  • Fyn proto-oncogene
  • FYN
  • fyn.S
  • fynb
  • fyn.L
  • Fyn
  • fyna
Epitope
AA 7-176
46
35
19
15
14
12
8
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
246
153
52
10
9
8
5
5
5
4
4
3
2
1
Host
Mouse
135
114
18
Clonality
Monoclonal
Conjugate
This FYN antibody is un-conjugated
9
9
9
9
8
7
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
252
126
68
50
32
29
28
27
5
4
4
2
2
2
2
Options
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Catalog No. ABIN94311
$ 316.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.972908 ABIN269149 ICC IF IHC (p) IP IHC WB Mouse IgG2b Log in to see FYN-01 4
1 ABIN2704810 IP IHC WB Rabbit C-Term, pTyr530 Log in to see Polyclonal 0
1 ABIN256460 IP WB Mouse IgG AA 7-176 Log in to see FYN-59 0
1 ABIN214218 IHC (p) IP WB Mouse IgG AA 7-176 Log in to see FYN-59 0
1 ABIN239824 ICC IHC (p) IP WB Mouse IgG AA 7-176 Log in to see FYN-01 0
1 ABIN1723376 ICC FACS IHC (p) IP WB Mouse IgG AA 7-176 Log in to see FYN-01 0
1 ABIN2469756 IP IHC WB Mouse IgG AA 7-176 Log in to see FYN-59 0
1 ABIN2469997 ICC IP IHC WB Mouse IgG AA 7-176 Log in to see FYN-01 0
1 ABIN2473714 IHC (fro) IP WB Mouse IgG2b AA 7-176 Log in to see FYN-01 4
1 ABIN1724003 IHC (p) IP WB Mouse IgG AA 7-176 Log in to see FYN-59 0
1 ABIN201639 ELISA IHC (fro) IHC (p) IP WB Mouse IgG1 AA 1-206 Log in to see 0
1 ABIN208495 Func IP ELISA WB Mouse IgG1, kappa AA 1-206 Log in to see 0
1 ABIN120706 IF IHC (p) IP WB Mouse IgG2b AA 7-176 Log in to see FYN-01 0
1 ABIN206566 IHC (fro) IP WB Mouse IgG AA 7-176 Log in to see 0
1 ABIN2628920 IHC (p) IP WB Rabbit pTyr530 Log in to see Polyclonal 0
1 ABIN4312920 ICC IF IHC IHC (p) IP WB PerCP Mouse IgG2b Log in to see FYN-01 0
1 ABIN3200170 ICC IHC (p) IP WB Mouse IgG2b Log in to see FYN-01 0
1 ABIN5550549 IP WB Mouse IgG AA 7-176, phosphorylated Log in to see FYN-59 0
1 ABIN4312921 ICC IF IHC IHC (p) IP WB APC Mouse IgG2b Log in to see FYN-01 0
1 ABIN4312919 ICC IF IHC IHC (p) IP WB PE Mouse IgG2b Log in to see FYN-01 0

General

Antigen FYN Oncogene Related To SRC, FGR, YES (FYN) Antibodies
Epitope AA 7-176
(46), (35), (19), (15), (14), (12), (8), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(246), (153), (52), (10), (9), (8), (5), (5), (5), (4), (4), (3), (2), (1)
Host Mouse
(135), (114), (18)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYN antibody is un-conjugated
(9), (9), (9), (9), (8), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(252), (126), (68), (50), (32), (29), (28), (27), (5), (4), (4), (2), (2), (2), (2)
Pubmed 10 references available
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Product Details anti-FYN Antibody

Target Details FYN Application Details Handling References for anti-FYN antibody (ABIN94311) Images
Specificity The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase.
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
Clone FYN-01
Isotype IgG2b

Target Details FYN

Product Details anti-FYN Antibody Application Details Handling References for anti-FYN antibody (ABIN94311) Images back to top
Antigen
Alternative Name FYN (FYN Antibody Abstract)
Background Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.
Pathways JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling

Application Details

Product Details anti-FYN Antibody Target Details FYN Handling References for anti-FYN antibody (ABIN94311) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FYN Antibody Target Details FYN Application Details References for anti-FYN antibody (ABIN94311) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-FYN antibody (ABIN94311)

Product Details anti-FYN Antibody Target Details FYN Application Details Handling Images back to top
Product cited in:

Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007

Background publications

Rivera, Olivera: "A current understanding of Fc epsilon RI-dependent mast cell activation." in: Current allergy and asthma reports, Vol. 8, Issue 1, pp. 14-20, 2008

Makinodan, Manabe, Makinodan, Yamauchi, Matsuyoshi, Sakumura, Tatsumi, Wanaka: "A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells." in: Neuroscience, Vol. 147, Issue 1, pp. 1-4, 2007

Samayawardhena, Kapur, Craig: "Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells." in: Blood, Vol. 109, Issue 9, pp. 3679-86, 2007

Filby, Seddon, Kleczkowska, Salmond, Tomlinson, Smida, Lindquist, Schraven, Zamoyska: "Fyn regulates the duration of TCR engagement needed for commitment to effector function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 7, pp. 4635-44, 2007

Wu, Hsu, Gleichman, Baconguis, Coulter, Lynch: "Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems." in: The Journal of biological chemistry, Vol. 282, Issue 28, pp. 20075-87, 2007

Hattori, Uchino, Isosaka, Maekawa, Iyo, Sato, Kohsaka, Yagi, Yuasa: "Fyn is required for haloperidol-induced catalepsy in mice." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7129-35, 2006

Talmor-Cohen, Tomashov-Matar, Tsai, Kinsey, Shalgi: "Fyn kinase-tubulin interaction during meiosis of rat eggs." in: Reproduction (Cambridge, England), Vol. 128, Issue 4, pp. 387-93, 2004

Utting, Priatel, Teh, Teh: "p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 3, pp. 1540-6, 2001

Images

Product Details anti-FYN Antibody Target Details FYN Application Details Handling References for anti-FYN antibody (ABIN94311) back to top
Supplier Images
Immunoprecipitation (IP) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (; gre...
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn
Immunofluorescence (IF) image for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (AA 7-176) antibody (ABIN94311) Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn (...